University of Pardubice, Faculty of Chemical Technology, Studentská 573, 532 10 Pardubice, Czech Republic.
University of Pardubice, Institute of Environmental and Chemical Engineering, Studentská 573, 530 12 Pardubice, Czech Republic.
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1366-74. doi: 10.1016/j.msec.2012.04.012. Epub 2012 Apr 18.
In this paper, the treatment of poly-ε-caprolactone (PCL) nano/micro-mesh system by cryogenic grinding and subsequent characterization of obtained product is described. The PCL nano/micro-mesh layer submerged in appropriate liquid was cryogenically ground and obtained particles were characterized employing mainly laser diffraction and scanning electron microscopy (SEM). In the ground sample, different types of particles (fibrous particles, fibrous fragments, agglomerates with and without an internal fibrous structure, lamellae and nanoparticles) were identified, described and quantified. Parameters of cryogenic grinding (weight of sample, type of liquid medium, and influence of sample storage) were optimized to maximize the yield of particles with desired features. The potential of the system for cell scaffolding was demonstrated by cultivation of 3T3 fibroblasts on the produced microparticles.
本文描述了通过低温粉碎处理聚己内酯（PCL）纳米/微网系统，并对所得产物进行了表征。将浸没在适当液体中的 PCL 纳米/微网层进行低温粉碎，然后主要采用激光衍射和扫描电子显微镜（SEM）对获得的颗粒进行表征。在粉碎后的样品中，鉴定出了不同类型的颗粒（纤维状颗粒、纤维状碎片、具有和不具有内部纤维结构的团聚体、薄片和纳米颗粒），并对其进行了描述和定量分析。优化了低温粉碎的参数（样品重量、液体介质类型以及样品储存的影响），以最大限度地提高具有所需特征的颗粒的产率。通过在制备的微颗粒上培养 3T3 成纤维细胞，证明了该系统用于细胞支架的潜力。
