附着有血小板的纳米纤维聚己内酯支架可刺激皮肤细胞增殖。
Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells.
作者信息
Vocetkova K, Buzgo M, Sovkova V, Bezdekova D, Kneppo P, Amler E
机构信息
Department of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, 150 06, Prague 5, Czech Republic.
Laboratory of Tissue Engineering, Institute of Experimental Medicine, Czech Academy of Sciences, 142 20, Prague 4, Czech Republic.
出版信息
Cell Prolif. 2016 Oct;49(5):568-78. doi: 10.1111/cpr.12276. Epub 2016 Jul 24.
OBJECTIVES
Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively.
METHODS
In this study, we introduced electrospun scaffolds composed of polycaprolactone (PCL) nanofibers functionalized with adhered platelets, as a source of numerous growth factors. Three concentrations of platelets were immobilized to nanofibrous scaffolds by simple adhesion, and their influence on adhesion, proliferation and metabolic activity of seeded cells (murine fibroblasts, keratinocytes and melanocytes) was investigated.
RESULTS
The data obtained indicated that presence of platelets significantly promoted cell spreading, proliferation and metabolic activity in all the skin-associated cell types. There were no significant differences among tested concentrations of platelets, thus even the lowest concentration sufficiently promoted proliferation of the seeded cells.
CONCLUSIONS
Such complex stimulation is needed for improved healing of chronic wounds. However, the nanofibrous system can be used not only as a skin cover, but also in broader applications in regenerative medicine.
目的
伤口愈合不良是一个全球性的医疗保健问题。慢性伤口的一般特征是生长因子可用性降低。正在开发新的策略以更有效地递送生长因子。
方法
在本研究中，我们引入了由聚己内酯（PCL）纳米纤维组成的电纺支架，该纳米纤维用粘附的血小板进行了功能化处理，作为多种生长因子的来源。通过简单的粘附将三种浓度的血小板固定在纳米纤维支架上，并研究了它们对接种细胞（小鼠成纤维细胞、角质形成细胞和黑素细胞）的粘附、增殖和代谢活性的影响。
结果
获得的数据表明，血小板的存在显著促进了所有皮肤相关细胞类型中的细胞铺展、增殖和代谢活性。在测试的血小板浓度之间没有显著差异，因此即使是最低浓度也足以促进接种细胞的增殖。
结论
慢性伤口的愈合改善需要这种复杂的刺激。然而，纳米纤维系统不仅可以用作皮肤覆盖物，还可以在再生医学中得到更广泛的应用。
Zotero 插件