Sevá-Pereira A, Berenhi P C, de Magalhães A F
Arq Gastroenterol. 1986 Apr-Jun;23(2):95-8.
Microscopical examination of stool fat is an easy laboratory procedure a long ago used as screening for steatorrhea. Quantitative analysis of stool fat is accepted as the best method to diagnose fat malabsorption, but stool collection and the laboratory technique are more difficult and time-consuming. The main objective of this work was performed to compare the accuracy of microscopical qualitative examination in the same fecal sample collected in 500 patients, 299 adults and 201 children, for the three days quantitative method. The diagnosis of steatorrhea was confirmed by chemical determination in 237 adults (stool fat values greater than 5 g) and 131 children (stool fat values greater than 2 g). From these 62 patients adults and 70 children were admitted as controls. The study showed that microscopical qualitative fat evaluation is highly comparable with findings obtained by chemical dosage. However, it is important to consider the use of two microscopic examination for each fecal sample, that must be carefully homogenized.
粪便脂肪的显微镜检查是一种简单的实验室操作,很久以前就被用作脂肪泻的筛查方法。粪便脂肪的定量分析被认为是诊断脂肪吸收不良的最佳方法,但粪便收集和实验室技术更困难且耗时。这项工作的主要目的是比较500例患者(299名成人和201名儿童)收集的同一粪便样本中显微镜定性检查与三天定量方法的准确性。通过化学测定确诊脂肪泻的有237名成人(粪便脂肪值大于5克)和131名儿童(粪便脂肪值大于2克)。从这些患者中,62名成人和70名儿童被纳入对照组。研究表明,显微镜下的定性脂肪评估与化学检测结果高度可比。然而,重要的是要考虑对每个粪便样本进行两次显微镜检查,且样本必须仔细均质化。