Amann S T, Josephson S A, Toskes P P
Department of Medicine, University of Florida, Gainesville 32610-0214, USA.
Am J Gastroenterol. 1997 Dec;92(12):2280-4.
The detection and evaluation of steatorrhea in a rapid, quantitative fashion are clinically needed in patients with suspected steatorrhea. Our aim was to evaluate the acid steatocrit method, on random spot stools in adults with and without steatorrhea, relative to the qualitative (microscopic) and quantitative assessments for fecal fat.
Stool samples were collected 72 h after a diet of 100 g of fat per day and randomly from 15 healthy controls, 14 patients with chronic pancreatitis, and seven patients with small bowel disease. All stools had quantitative, qualitative, and acid steatocrit analyses performed for fecal fat.
The sensitivity and specificity for the detection of steatorrhea by the spot stool qualitative fecal fat were 78 and 70%, respectively. The spot stool acid steatocrit correlated linearly with the 72-h stool quantitative fecal fat (g/24 h), r = 0.761 and p < 0.001. The acid steatocrit on random spot stools, compared with the 72-h stool quantitative fecal fat, revealed a sensitivity of 100%, a specificity of 95%, and a positive predictive value of 90% for the detection of steatorrhea. It also estimated the quantitative fecal fat.
The acid steatocrit can be performed accurately on random spot stools and can be used to detect the presence of steatorrhea and estimate the quantitative fecal fat. This assay can be done with readily available equipment for rapid evaluation. Use of a spot stool sample simplifies the acid steatocrit, further improving on the practicality of this test. This study also confirms the clinical usefulness of this simplified method to detect steatorrhea.
临床上需要以快速、定量的方式对疑似脂肪泻患者进行脂肪泻的检测和评估。我们的目的是评估酸脂肪细胞比容法在有或无脂肪泻的成人随机粪便样本中的应用，并与粪便脂肪的定性（显微镜检查）和定量评估进行比较。
在每天摄入100克脂肪的饮食72小时后，从15名健康对照者、14名慢性胰腺炎患者和7名小肠疾病患者中随机采集粪便样本。对所有粪便进行粪便脂肪的定量、定性和酸脂肪细胞比容分析。
粪便定性脂肪检测脂肪泻的敏感性和特异性分别为78%和70%。随机粪便样本的酸脂肪细胞比容与72小时粪便定量脂肪（克/24小时）呈线性相关，r = 0.761，p < 0.001。与72小时粪便定量脂肪相比，随机粪便样本的酸脂肪细胞比容检测脂肪泻的敏感性为100%，特异性为95%，阳性预测值为90%。它还能估计粪便脂肪的量。
酸脂肪细胞比容可在随机粪便样本上准确进行，可用于检测脂肪泻的存在并估计粪便脂肪的量。该检测可使用现成的设备进行快速评估。使用粪便样本简化了酸脂肪细胞比容检测，进一步提高了该检测的实用性。本研究还证实了这种简化方法在检测脂肪泻方面的临床实用性。
