Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng District, Beijing, 100730, China.
Neurol Sci. 2014 Jun;35(6):831-7. doi: 10.1007/s10072-013-1605-4. Epub 2013 Dec 24.
Recent studies showed that hyperglycemia is the main trigger of diabetic cognitive impairment and can cause hippocampus abnormalities. The goal of this study is to explore the effects of different concentrations of high glucose for different exposure time on cell viability as well as intracellular reactive oxygen species (ROS) generation of primary cultured hippocampal neurons. Hippocampal neurons were exposed to different concentrations of high glucose (50, 75, 100, 125, and 150 mM) for 24, 48, 72 and 96 h. Cell viability and nuclear morphology were evaluated by MTT and Hoechst assays, respectively. Intracellular ROS were monitored using the fluorescent probe DCFH-DA. The results showed that, compared with control group, the cell viability of all high glucose-treated groups decreased significantly after 72 h and there also was a significant increase of apoptotic nuclei in high glucose-treated groups from 72 to 96 h. Furthermore, 50 mM glucose induced a peak rise in ROS generation at 24 h and the intracellular ROS levels of 50 mM glucose group were significantly higher than the corresponding control group from 6 to 72 h. These results suggest that hippocampal neurons could be injured by high glucose exposure and the neuronal injury induced by high glucose is potentially mediated through intracellular ROS accumulation.
最近的研究表明，高血糖是导致糖尿病认知障碍的主要诱因，可引起海马体异常。本研究旨在探讨不同浓度高葡萄糖作用于原代培养海马神经元不同时间对细胞活力及细胞内活性氧（ROS）生成的影响。将海马神经元分别暴露于不同浓度的高葡萄糖（50、75、100、125 和 150mM）24、48、72 和 96h。通过 MTT 和 Hoechst 测定法分别评估细胞活力和核形态。使用荧光探针 DCFH-DA 监测细胞内 ROS。结果表明，与对照组相比，所有高葡萄糖处理组在 72h 后细胞活力明显下降，高葡萄糖处理组从 72h 到 96h 凋亡核明显增加。此外，50mM 葡萄糖在 24h 时诱导 ROS 生成的峰值升高，并且 50mM 葡萄糖组的细胞内 ROS 水平从 6h 到 72h 均显著高于相应的对照组。这些结果表明，海马神经元可能受到高葡萄糖暴露的损伤，高葡萄糖诱导的神经元损伤可能通过细胞内 ROS 积累介导。
