Troianovskiĭ S M, Gleĭberman A S, Bannikov G A
Biull Eksp Biol Med. 1987 Apr;103(4):478-81.
It has been shown by indirect immunofluorescence using monoclonal antibodies against adult rat simple epithelial prekeratins with the molecular weight of 55 kD (PK55) and 40 kD (PK40) that PK55 was expressed in the covering ectoderm until the 11th day of the antenatal period. PK55 expression markedly increased in ectodermal cells lining the heart region, with PK40 appearing in the same cells on day 11. Beginning from the 16th day gradual loss of both prekeratins started with the parallel formation of squamous differentiated epidermis. These proteins were retained in the outer layers of peridermal cells and in some cells of the basal layer (probably Merkel cells). PK55 was re-expressed on the 18th day in cells migrating into dermal layer during hair folliculi formation. PK55 disappeared again in mature folliculi. Thus, a correlation between distinct morphogenetic events and PK55 and PK40 expression has been found.
使用针对成年大鼠分子量为55kD（PK55）和40kD（PK40）的简单上皮前角蛋白的单克隆抗体进行间接免疫荧光检测，结果显示PK55在产前第11天之前在覆盖外胚层中表达。PK55在心脏区域内衬的外胚层细胞中表达明显增加，PK40在第11天出现在相同细胞中。从第16天开始，两种前角蛋白逐渐消失，同时平行形成鳞状分化表皮。这些蛋白质保留在周皮细胞外层和基底层的一些细胞（可能是默克尔细胞）中。在毛囊形成过程中迁移到真皮层的细胞中，PK55在第18天重新表达。PK55在成熟毛囊中再次消失。因此，已发现不同形态发生事件与PK55和PK40表达之间存在相关性。
