Inenaga K, Mason W T
Brain Res. 1987 Mar 3;405(1):159-64. doi: 10.1016/0006-8993(87)91001-8.
The effect of gamma-aminobutyric acid (GABA) on normal bovine lactotrophs was investigated using the patch clamp technique. GABA application (1-10 microM) induced fluctuations of membrane current, accompanied by an increase in inward current. The mean conductance of GABA-activated channels was estimated to be 19 pS from statistical analysis of these fluctuations whereas the mean conductance for GABA-activated single ion channels was 16 pS. The reversal potential of the current was near to the equilibrium potential for chloride ions. These results indicate that the one possible mechanism for inhibition of prolactin release induced by GABA is probably mediated by activation of chloride channels.
采用膜片钳技术研究了γ-氨基丁酸（GABA）对正常牛催乳素细胞的作用。施加GABA（1 - 10微摩尔）可诱导膜电流波动，并伴有内向电流增加。通过对这些波动的统计分析，GABA激活通道的平均电导估计为19皮西门子，而GABA激活的单离子通道的平均电导为16皮西门子。电流的反转电位接近氯离子的平衡电位。这些结果表明，GABA诱导催乳素释放受抑制的一种可能机制可能是由氯离子通道的激活介导的。
