Marchetti E, Querzoli P, Moncharmont B, Parikh I, Bagni A, Marzola A, Fabris G, Nenci I
Cancer Res. 1987 May 1;47(9):2508-13.
Immunocytochemical demonstration of estrogen receptors in 115 human breast cancer specimens was performed using mouse monoclonal antibodies against estrogen receptor and avidin-biotin as the displaying system. The antibody indicated a highly heterogeneous endowment of neoplastic cells with estrogen receptor at both nuclear and cytoplasmic levels. The percentage of labeled cells within each tumor specimen was recorded to compare this immunocytochemical assay with the biochemical assay of estrogen receptors by the dextran-coated charcoal method. A significant correlation was observed between these two assays. The present results show that estrogen receptors can be confidently demonstrated at the single cell level, thus providing additional information to quantitative biochemical assays. Their prognostic and therapeutic predictive powers may be usefully integrated, particularly in view of the heterogeneous distribution of receptors among cancer cells.
使用抗雌激素受体的小鼠单克隆抗体及抗生物素蛋白-生物素作为显示系统，对115份人乳腺癌标本进行雌激素受体的免疫细胞化学检测。该抗体显示肿瘤细胞在核水平和胞质水平均有高度异质性的雌激素受体禀赋。记录每个肿瘤标本中标记细胞的百分比，以便将这种免疫细胞化学检测与用葡聚糖包被活性炭法进行的雌激素受体生化检测相比较。观察到这两种检测之间存在显著相关性。目前的结果表明，雌激素受体可在单细胞水平得到可靠显示，从而为定量生化检测提供额外信息。它们的预后和治疗预测能力可能会得到有效整合，尤其是考虑到癌细胞中受体的异质性分布。
