Toi M, Hamada Y, Seto Y, Hisamatsu K, Suehiro S, Toge T, Niimoto M, Hattori T
Gan To Kagaku Ryoho. 1986 Oct;13(10):3056-62.
Estrogen receptor (ER) was measured by the estrogen receptor-immunocytochemical assay (ER-ICA) using monoclonal antibody in 36 mammary carcinomas. Simultaneously, ER and progesterone receptor (PgR) were measured by the dextran coated charcoal (DCC) method. In ER-ICA, tumor cells with estrogen receptors were histologically observed. The proportion of ER-positive tumor cells among tumor tissues and the levels of staining intensity were also examined. Results were compared to those obtained by the dextran coated charcoal (DCC) method. Tumors were evaluated as ER-ICA-positive when they contained more than 10% ER-positive cells. ER at a level of more than 5.0 fmol/mg protein was evaluated ER-positive and PgR at more than 5.0 fmol/mg protein as PgR-positive by the DCC method. Twenty-one out of 24 ER-ICA-positive tumors were ER-positive by the DCC method, and 11 out of 12 ER-ICA-negative tumors were ER-negative by the DCC method. Correlation of ER-ICA and ER by the DCC method was 88.9%. There were 11 PgR-positive tumors, and all were included among ER-ICA-positive tumors which had a high proportion of ER-positive cells and high staining intensities.
采用单克隆抗体，通过雌激素受体免疫细胞化学检测法（ER-ICA）对36例乳腺癌进行雌激素受体（ER）检测。同时，采用葡聚糖包被活性炭（DCC）法检测ER和孕激素受体（PgR）。在ER-ICA检测中，对具有雌激素受体的肿瘤细胞进行组织学观察。还检测了肿瘤组织中ER阳性肿瘤细胞的比例及染色强度水平。将结果与通过葡聚糖包被活性炭（DCC）法获得的结果进行比较。当肿瘤中ER阳性细胞超过10%时，判定为ER-ICA阳性。通过DCC法，ER水平超过5.0 fmol/mg蛋白判定为ER阳性，PgR超过5.0 fmol/mg蛋白判定为PgR阳性。24例ER-ICA阳性肿瘤中，21例通过DCC法检测为ER阳性；12例ER-ICA阴性肿瘤中，11例通过DCC法检测为ER阴性。ER-ICA与DCC法检测ER的相关性为88.9%。有11例PgR阳性肿瘤，均包含在ER-ICA阳性肿瘤中，这些肿瘤具有高比例的ER阳性细胞和高染色强度。
