Dept. of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India.
Dept. of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India.
Res Vet Sci. 2014 Feb;96(1):25-7. doi: 10.1016/j.rvsc.2013.12.001. Epub 2013 Dec 12.
Biofilm formation is one of the factors responsible for antibiotic resistance. The involvement of biofilm formation in bacterial mastitis is well known. Milk composition varies during the lactation period and certain pathogens are producing more number of mastitis cases during particular periods of lactation. The present study elucidates the effects of different milk components on biofilm formation and the persistence of infection. The Plackett Burman screening design has been chosen for assessing the significance. Biofilm production of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were assessed by crystal violet assay. Dipotassium hydrogen phosphate had a significant effect on biofilm formation by S. aureus (MTCC 1430) whereas it was pH in the case of biofilm formation by P. aeruginosa (NCIM 5029). Other independent factors were found to be insignificant.
生物膜的形成是导致抗生素耐药性的因素之一。生物膜的形成与细菌性乳腺炎有关，这一点已得到广泛认可。在泌乳期，牛奶成分会发生变化，某些病原体在泌乳期的特定阶段会导致更多的乳腺炎病例。本研究阐明了不同牛奶成分对生物膜形成和感染持续存在的影响。本研究采用 Plackett-Burman 筛选设计来评估其重要性。采用结晶紫法评估金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的生物膜生成情况。磷酸二氢钾对金黄色葡萄球菌（MTCC 1430）的生物膜形成有显著影响，而 pH 对铜绿假单胞菌（NCIM 5029）的生物膜形成有显著影响。其他独立因素则被认为是无足轻重的。
