补体激活在 Fuchs 角膜营养不良发病机制中的作用。

The role of complement activation in the pathogenesis of Fuchs' dystrophy.

机构信息

Department of Ophthalmology, Semmelweis University, 1085, Mária u. 39, Budapest, Hungary.

3rd Department of Internal Medicine, Research Laboratory, Semmelweis University, 1125, Kútvölgyi út 4, Budapest, Hungary.

出版信息

Mol Immunol. 2014 Apr;58(2):177-81. doi: 10.1016/j.molimm.2013.11.019. Epub 2013 Dec 22.

DOI:10.1016/j.molimm.2013.11.019

PMID:24368317

Abstract

PURPOSE

Inflammation can be an etiologic factor of Fuchs' dystrophy according to previous studies. Our aim was to analyse the activation of the complement system in the aqueous humor in this pathological condition.

METHODS

100 μl aqueous humor sample was taken during keratoplasty of 11 Fuchs' dystrophic patients and during phacoemulsification surgery of 18 control patients. The samples were mixed with EDTA and stored at -80 °C. Concentrations of C1rC1sC1Inh and C3bBbP complexes as markers of the activation of the classical and alternative complement pathways, respectively, were measured with ELISA method. The results of the patient group and the control group were compared with statistical analysis (non-parametric Mann Whitney test).

RESULTS

Both the concentrations of C1rC1sC1Inh [4.3 (3.2-20.2)AU/ml] and of C3bBbP [15.3 (7.8-22.6)AU/ml] were significantly higher in the Fuchs' dystrophic group than in the control group [C1rC1sC1Inh: 0.0 (0.0-5.6)AU/ml, C3bBbP: 1.4 (0.0-7.8)AU/ml]. The median value is shown along with the (25% and 75% percentiles).

CONCLUSIONS

Based on our results, the complement system may be activated both through the classical and alternative pathways in the aqueous humor of the patients with Fuchs' dystrophy.

摘要

目的

根据先前的研究，炎症可能是 Fuchs 角膜营养不良的病因之一。我们的目的是分析这种病理情况下房水中补体系统的激活情况。

方法

在 11 例 Fuchs 角膜营养不良患者的角膜移植术和 18 例对照患者的超声乳化手术中，分别采集 100μl 房水样本。将样本与 EDTA 混合并储存在-80°C。采用 ELISA 法测定经典和替代补体途径激活的标志物 C1rC1sC1Inh 和 C3bBbP 复合物的浓度。用统计分析（非参数曼-惠特尼检验）比较患者组和对照组的结果。

结果

Fuchs 角膜营养不良组的 C1rC1sC1Inh [4.3（3.2-20.2）AU/ml] 和 C3bBbP [15.3（7.8-22.6）AU/ml] 浓度均明显高于对照组 [C1rC1sC1Inh：0.0（0.0-5.6）AU/ml，C3bBbP：1.4（0.0-7.8）AU/ml]。中位数显示在（25%和 75%分位数）之间。