意大利中部男男性行为人群中HIV-1 CRF02_AG的传播：一项基于分子流行病学的研究。
Circulation of HIV-1 CRF02_AG among MSM population in central Italy: a molecular epidemiology-based study.
作者信息
Giuliani Massimo, Santoro Maria M, Lo Presti Alessandra, Cella Eleonora, Scognamiglio Paola, Lai Alessia, Latini Alessandra, Fabeni Lavinia, Gori Caterina, Pinnetti Carmela, Girardi Enrico, Perno Carlo F, Zehender Gianguglielmo, Ciccozzi Massimo
机构信息
Department of Infectious Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy ; HIV/AIDS Unit, San Gallicano Dermatological Institute (IRCCS), Rome, Italy.
University of Rome Tor Vergata, Rome, Italy.
出版信息
Biomed Res Int. 2013;2013:810617. doi: 10.1155/2013/810617. Epub 2013 Nov 28.
INTRODUCTION
The evolutionary and demographic history of the circular recombinant form CRF02_AG in a selected retrospective group of HIV-1 infected men who have sex with men (MSM) resident in Central Italy was investigated.
METHODS
A total of 55 HIV-1 subtype CRF02_AG pol sequences were analyzed using Bayesian methods and a relaxed molecular clock to reconstruct their dated phylogeny and estimate population dynamics.
RESULTS
Dated phylogeny indicated that the HIV-1 CRF02_AG strains currently circulating in Central Italy originated in the early 90's. Bayesian phylogenetic analysis revealed the existence of a main HIV-1 CRF02_AG clade, introduced in the area of Rome before 2000 and subsequently differentiated in two different subclades with a different date of introduction (2000 versus 2005). All the sequences within clusters were interspersed, indicating that the MSM analyzed form a close and restricted network where the individuals, also moving within different clinical centers, attend the same places to meet and exchange sex.
CONCLUSIONS
It was suggested that the HIV-1 CRF02_AG epidemic entered central Italy in the early 1990s, with a similar trend observed in western Europe.
引言
对居住在意大利中部的一组选定的男男性行为者（MSM）感染HIV-1的回顾性队列中，环状重组形式CRF02_AG的进化和人口统计学历史进行了调查。
方法
使用贝叶斯方法和宽松分子钟对总共55个HIV-1亚型CRF02_AG pol序列进行分析，以重建其带时间信息的系统发育并估计种群动态。
结果
带时间信息的系统发育表明，目前在意大利中部流行的HIV-1 CRF02_AG毒株起源于90年代初。贝叶斯系统发育分析显示存在一个主要的HIV-1 CRF02_AG进化枝，于2000年前引入罗马地区，随后分化为两个不同的亚进化枝，引入时间不同（2000年与2005年）。簇内的所有序列相互穿插，表明所分析的男男性行为者形成了一个紧密且有限的网络，其中个体即使在不同临床中心活动，也会前往相同场所进行会面和性交流。
结论
提示HIV-1 CRF02_AG疫情于20世纪90年代初传入意大利中部，这一趋势在西欧也有观察到。
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