重组人β干扰素增强兔内毒素发热反应

Augmentation of endotoxin fever by recombinant human beta interferon in rabbits.

作者信息

Kawasaki H, Moriyama M, Tanaka A

出版信息

Infect Immun. 1987 May;55(5):1121-5. doi: 10.1128/iai.55.5.1121-1125.1987.

DOI:10.1128/iai.55.5.1121-1125.1987

PMID:2437032

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC260478/

Abstract

Nonpyrogenic amounts of endotoxin (0.1 to 1 ng/kg), hardly detectable by conventional Limulus amoebocyte lysate tests, could produce a fever of around 1 degree C when injected with a nonpyrogenic dose (6 X 10(5) U/kg) of recombinant human beta interferon (IFN-beta) in rabbits. Release of endogenous IFN and tumor necrosis factor by endotoxin was also dramatically increased by recombinant human IFN-beta, and their levels in the blood were closely correlated with the increase of body temperature. These data suggest, if the synergism between IFN and endotoxin also operates in the homologous system (human IFN-human cells), that contaminating endotoxin in IFNs, even if not detectable by Limulus amoebocyte lysate test, can contribute to IFN fever to a considerable extent in humans.

摘要

非致热剂量的内毒素（0.1至1纳克/千克），用传统鲎试剂试验几乎检测不到，当与非致热剂量（6×10⁵单位/千克）的重组人β干扰素（IFN-β）一起注射到兔子体内时，可引起约1摄氏度的发热。重组人IFN-β还显著增加了内毒素对内源性IFN和肿瘤坏死因子的释放，并且它们在血液中的水平与体温升高密切相关。这些数据表明，如果IFN与内毒素之间的协同作用也在同源系统（人IFN-人细胞）中起作用，那么IFN中污染的内毒素，即使鲎试剂试验检测不到，在人类中也可能在很大程度上导致IFN发热。