与DQw1相关的两种DQβ等位基因特异性的同种异体反应性T细胞识别。

Alloreactive T-cell recognition of two DQ beta allelic specificities associated with DQw1.

作者信息

Zeevi A, Cascino I, Di Vecchia L, Marrari M, Duquesnoy R J, Trucco M

出版信息

Hum Immunol. 1987 Mar;18(3):225-34. doi: 10.1016/0198-8859(87)90087-5.

Abstract

Two sets of alloreactive T-cell clones from different original mixed lymphocyte reactions (MLR) discriminate between two DQ beta allelic forms when these are expressed in association with the same DQ alpha form. From these results we concluded that it is possible to guide a DQ specific response of alloreactive T-cell clones by setting up an appropriate responder-stimulator combination in the original MLR; DQw1 is associated with two allelic forms of the DQ molecule recognized as lymphocyte activating determinants by alloreactive T-cell clones; alloreactive T cells recognize specific alpha-beta chain combination of the DQ molecule; the allelic forms that differentiate the alpha or the beta chains of the DQ molecule from each other can also be recognized at the molecular level by DQ alpha and DQ beta RFLP analysis.

摘要

当两种DQβ等位基因形式与相同的DQα形式共同表达时，来自不同原始混合淋巴细胞反应（MLR）的两组同种异体反应性T细胞克隆能够区分这两种形式。从这些结果我们得出结论，通过在原始MLR中建立合适的反应者-刺激者组合，有可能引导同种异体反应性T细胞克隆产生DQ特异性反应；DQw1与DQ分子的两种等位基因形式相关，这两种形式被同种异体反应性T细胞克隆识别为淋巴细胞激活决定簇；同种异体反应性T细胞识别DQ分子特定的α-β链组合；通过DQα和DQβ限制性片段长度多态性（RFLP）分析，在分子水平上也能够识别使DQ分子的α链或β链相互区分的等位基因形式。