Larsen C, Johansen M
J Chromatogr. 1987 Feb 27;389(1):227-35. doi: 10.1016/s0021-9673(01)94426-7.
A high-performance size-exclusion chromatography procedure using Nucleosil Diol has been developed which provides the simultaneous determination of macromolecular dextran metronidazole monosuccinate ester prodrugs and the hydrolysis products metronidazole and metronidazole monosuccinate. Various factors influencing the chromatographic behaviour of the compounds are discussed. Baseline separation of the three substances was achieved within 8 min by using a 0.05 M phosphate buffer pH 7.5 eluent at a flow-rate of 2 ml min-1. The detection limit at 320 nm for a conjugate with a degree of substitution of 4.61 was found to be 3.5 micrograms ml-1.
已开发出一种使用Nucleosil二醇的高效尺寸排阻色谱法，可同时测定大分子葡聚糖甲硝唑单琥珀酸酯前药以及水解产物甲硝唑和甲硝唑单琥珀酸酯。讨论了影响这些化合物色谱行为的各种因素。通过使用pH 7.5的0.05 M磷酸盐缓冲液作为洗脱液，流速为2 ml min-1，在8分钟内实现了三种物质的基线分离。对于取代度为4.61的缀合物，在320 nm处的检测限为3.5微克ml-1。
