Ali Nouruddin Wageih, Gamal Mohammed, Abdelkawy Mohammed
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy St., Beni-Suef Egypt.
Pak J Pharm Sci. 2013 Sep;26(5):865-71.
Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline (DIHQ) and metronidazole (MTN). In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid (7.5: 2.5: 0.1, by volume) as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water : methanol (60 :40, V/V, PH=3.6 )as mobile phase at a flow rate of 0.7 mL.min-1 and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation.
开发了两种色谱方法用于分析二碘羟基喹啉（DIHQ）和甲硝唑（MTN）。在第一种方法中，二碘羟基喹啉和甲硝唑在硅胶60F254板上进行TLC分离，以氯仿：丙酮：冰醋酸（体积比7.5:2.5:0.1）作为流动相。然后在254nm处扫描得到的条带。第二种方法是RP-HPLC法，其中二碘羟基喹啉和甲硝唑在反相C18柱上分离，以水：甲醇（60:40，V/V，pH = 3.6）作为流动相，流速为0.7 mL·min-1，并在220nm处进行紫外检测。上述方法已成功用于测定纯形式及其药物制剂中的二碘羟基喹啉和甲硝唑。
