Automated separation and measurement of urinary isoenzymes and protein by ion-exchange liquid chromatography.

This paper deals with a totally automated detection system for the assay of urinary isoenzymes and protein using high-performance liquid chromatography with a continuous post-column detection system. We attempted to determine the distribution of three enzymes in urine samples from a healthy child and in tissue extracts of rabbits. Alkaline phosphatase, gamma-glutamyltransferase and lactate dehydrogenase isoenzymes were each separated into six peaks. In comparison with the previous methods, this procedure provides better precision and accuracy, and it is sufficiently sensitive to allow the analysis without preconcentration of urine samples.