Jeong Ji-Min, Kim Ju-Won, Park Hyoung-Jun, Song Jeong-Hun, Kim Do-Hyung, Park Chan-Il
Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
The College of Fisheries Science, Pukyong National University, Busan 608-737, Republic of Korea.
Results Immunol. 2011 Jul 14;1(1):11-7. doi: 10.1016/j.rinim.2011.06.002. eCollection 2011.
Fas belongs to the tumour necrosis factor (TNF) receptor superfamily and can transmit a death signal leading to apoptosis. In the present study, we isolated the full-length cDNA for rock bream (Oplegnathus fasciatus) Fas (RbFas). The full-length RbFas cDNA was 1770 bp long and contained an open reading frame of 957 bp that encoded 319 amino acid residues with a predicted molecular mass of 35.1 kDa. The 319 amino-acid predicted RbFas sequence is homologous to other Fas sequences, contains three cysteine-rich domains and a death domain (DD) and two potential N-glycosylation sites. Expression of RbFas mRNA was detected in nine different tissues from healthy rock bream and was the highest in red blood cells. In analyses of mitogen-stimulated RbFas expression in peripheral blood leucocytes, expression of RbFas mRNA was observed between 1 and 36 h after stimulation with LPS, and 1 and 3 h stimulation with poly I:C. In the case of bacterial injection, the RbFas transcript peaked 6 h after injection in both the kidney and the spleen. Otherwise, the RbFas transcript peaked after 1 h in spleen and 6 h in kidney following injection with RSIV.
Fas属于肿瘤坏死因子（TNF）受体超家族，可传递导致细胞凋亡的死亡信号。在本研究中，我们分离出了条石鲷（Oplegnathus fasciatus）Fas（RbFas）的全长cDNA。RbFas cDNA全长1770 bp，包含一个957 bp的开放阅读框，编码319个氨基酸残基，预测分子量为35.1 kDa。预测的319个氨基酸的RbFas序列与其他Fas序列同源，包含三个富含半胱氨酸的结构域、一个死亡结构域（DD）和两个潜在的N-糖基化位点。在健康条石鲷的九个不同组织中检测到RbFas mRNA的表达，在红细胞中表达最高。在对有丝分裂原刺激的外周血白细胞中RbFas表达的分析中，在用LPS刺激后1至36小时以及用聚肌胞苷酸刺激1至3小时观察到RbFas mRNA的表达。在细菌注射的情况下，RbFas转录本在注射后6小时在肾脏和脾脏中均达到峰值。否则，注射RSIV后，RbFas转录本在脾脏中1小时后达到峰值，在肾脏中6小时后达到峰值。
