Kim Ju-Won, Lee Jung-Ho, Bae Jin-Sol, An Cheul Min, Nam Bo-Hye, Jeong Ji-Min, Park Chan-Il
Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong 650-160, Republic of Korea.
Inland Fisheries Research Institute, NFRDI, Jinhae 645-805, Republic of Korea.
Dev Comp Immunol. 2015 Sep;52(1):69-74. doi: 10.1016/j.dci.2015.04.012. Epub 2015 Apr 28.
Thioredoxin-interacting protein (TXNIP) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. The full-length rock bream (Oplegnathus fasciatus) TXNIP (RbTXNIP) cDNA (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. Furthermore, multiple alignments showed that the arrestin domain was well conserved among the other TXNIP sequences tested. RbTXNIP was predicted to contain a PxxP and PPxY motif. Phylogenetic analysis indicated that RbTXNIP is most closely related to Fugu rubripes TXNIP. RbTXNIP was expressed significantly in the RBC, intestine, and spleen. RbTXNIP mRNA expression was also examined in several tissues under conditions of bacterial and viral challenge. Generally, all tissues examined from fish infected with Streptococcus iniae, Edwardsiella tarda and red sea bream iridovirus (RSIV) showed significant downregulation in RbTXNIP expression compared to controls. However, RbTXNIP expression showed significant upregulation in the spleen and kidney after injection of recombinant rock bream TRx1 protein. These findings provide a molecular foundation for functional studies and applications in teleosts.
硫氧还蛋白相互作用蛋白（TXNIP）是葡萄糖代谢的重要调节因子，通过抑制细胞对葡萄糖的摄取发挥作用。全长条石鲷（Oplegnathus fasciatus）TXNIP（RbTXNIP）cDNA（2499 bp）包含一个1188 bp的开放阅读框，编码396个氨基酸。此外，多重比对显示，在所测试的其他TXNIP序列中，抑制蛋白结构域高度保守。预测RbTXNIP含有一个PxxP和PPxY基序。系统发育分析表明，RbTXNIP与红鳍东方鲀TXNIP关系最为密切。RbTXNIP在红细胞、肠道和脾脏中显著表达。还在细菌和病毒攻击条件下检测了几种组织中的RbTXNIP mRNA表达。一般来说，与对照组相比，感染海豚链球菌、迟缓爱德华氏菌和真鲷虹彩病毒（RSIV）的鱼的所有检测组织中，RbTXNIP表达均显著下调。然而，注射重组条石鲷TRx1蛋白后，脾脏和肾脏中的RbTXNIP表达显著上调。这些发现为硬骨鱼的功能研究和应用提供了分子基础。
