Singh Anurag, Holvoet Sébastien, Weiss Marietta, Beaumont Maurice, Zuercher Adrian W, Mercenier Annick
Allergy Group, Department of Nutrition & Health, Nestle Research Center, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland.
Clinical Evaluation Group, Department of Bio-Analytical Science, Nestle Research Center, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland.
Results Immunol. 2011 Jul 27;1(1):18-23. doi: 10.1016/j.rinim.2011.07.002. eCollection 2011.
There is a need for simple and physiological assays to characterize the immune status of allergic individuals. Whole blood samples from 15 adult subjects (10 with positive clinical history to grass pollen and 5 with negative clinical history) were obtained before the start (April 2010) and during the middle of the grass pollen season (June 2010). The investigators were blinded to the allergic status of the subjects. A skin prick test (SPT) to grass pollen was carried out at the end of the study. Cytokines (IL-5, IL-13, IL-10 and IFNγ) and activation of T-lymphocytes were determined after ex vivo culture of whole blood cells. IL-5, IL-10 and IL-13 cytokines were significantly elevated in allergic individuals during the middle of the season (p≤0.02) compared to the start. This assay can be a valuable tool in clinical trials especially in pediatric population where limited quantities of blood are available to study immune responses.
需要有简单且符合生理情况的检测方法来表征过敏个体的免疫状态。在草花粉季节开始前（2010年4月）和季节中期（2010年6月），采集了15名成年受试者的全血样本（其中10名有草花粉临床阳性病史，5名有临床阴性病史）。研究人员对受试者的过敏状态不知情。在研究结束时进行了草花粉皮肤点刺试验（SPT）。对全血细胞进行体外培养后，测定细胞因子（IL-5、IL-13、IL-10和IFNγ）以及T淋巴细胞的活化情况。与季节开始时相比，过敏个体在季节中期IL-5、IL-10和IL-13细胞因子显著升高（p≤0.02）。该检测方法在临床试验中可能是一种有价值的工具，尤其是在儿科人群中，因为可用于研究免疫反应的血量有限。
