IRIBHM, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
Laboratory of Physiology, School of Medicine, ULB, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, Belgium.
FEBS Open Bio. 2013 Dec 7;4:55-9. doi: 10.1016/j.fob.2013.11.007. eCollection 2013.
NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2 (.-) and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor. It is shown here that DPI, at concentrations similar to those which inhibit the generation of O2 derivatives, activated the efflux of radioiodide but not of its analog (99m)TcO4 (-) nor of the K(+) cation mimic (86)Rb(+) in thyroid cells, in the PCCl3 rat thyroid cell line and in COS cell lines expressing the iodide transporter NIS. Effects obtained with DPI, especially in thyroid cells, should therefore be interpreted with caution.
NADPH 氧化酶（NOXes）和双氧化酶（DUOXes）产生 O2（.-）和 H2O2。二苯基碘鎓（DPI）抑制这些酶的活性，通常被用作特异性抑制剂。本文显示，在与抑制 O2 衍生物生成相似的浓度下，DPI 激活了放射性碘的外排，但不能激活其类似物（99m）TcO4（-）或 K（+）阳离子模拟物（86）Rb（+）在甲状腺细胞、PCCl3 大鼠甲状腺细胞系和表达碘转运蛋白 NIS 的 COS 细胞系中。因此，应该谨慎解释 DPI 获得的效果，特别是在甲状腺细胞中。
