1 Department of Otorhinolaryngology, University Medical Center Ulm , Ulm, Germany .
Tissue Eng Part A. 2014 Jun;20(11-12):1668-78. doi: 10.1089/ten.TEA.2013.0365. Epub 2014 Feb 6.
Tissue engineering is considered a promising future option for nasal cartilage repair. However, until now, an optimal material has not been identified for this specific purpose. Therefore, the aim of this study was to analyze a recently developed decellularized collagen matrix, which has promising material properties for septal cartilage repair. A tetrazolium dye based cytotoxicity assay using rat nasal septum chondrocytes was performed to examine the cytotoxic effects of decellularized cartilage matrices. Unseeded scaffolds as well as scaffolds seeded with chondrocytes were implanted in nasal septum defects in Lewis rats to investigate the cellular and humoral inflammatory responses in the surrounding tissue as well as the effect on the formation of nasal septum perforations. Samples were analyzed histochemically and immunohistochemically after 1, 4, and 12 weeks. Although cells for the cytotoxicity assay were cultured under serum-free conditions for 24 h to increase sensitivity, no cytotoxic effects were detected. Histological and immunohistochemical evidence displayed that the implanted scaffolds induced minor macrophage and lymphocyte infiltration and were well integrated at the contact site to native cartilage and between the mucosal membranes. The biocompatibility index revealed only slightly irritating effects during the study period. Septal perforations were prevented efficiently. In summary, our results provide evidence that decellularized xenogeneic collagen scaffolds are suitable for cartilage tissue engineering. The scaffolds were integrated well into septal cartilage defects without causing a strong inflammatory reaction and prevented the development of nasal septum perforations. Therefore, we envision the possibility to use them in nasal cartilage repair in the future.
组织工程被认为是一种有前途的未来选择，可用于修复鼻软骨。然而，到目前为止，还没有确定一种针对这一特定用途的最佳材料。因此，本研究旨在分析一种最近开发的脱细胞胶原基质，该基质具有修复鼻中隔软骨的有前途的材料特性。使用大鼠鼻中隔软骨细胞进行四唑盐染料基于细胞毒性的测定，以检查脱细胞软骨基质的细胞毒性作用。未接种细胞的支架以及接种细胞的支架被植入Lewis 大鼠鼻中隔缺损中，以研究周围组织中的细胞和体液炎症反应以及对鼻中隔穿孔形成的影响。在 1、4 和 12 周后，对样本进行组织化学和免疫组织化学分析。尽管细胞毒性测定中细胞在无血清条件下培养 24 小时以增加敏感性，但未检测到细胞毒性作用。组织学和免疫组织化学证据显示，植入的支架引起了少量巨噬细胞和淋巴细胞浸润，并与天然软骨和粘膜之间的接触部位很好地整合。生物相容性指数仅在研究期间显示出轻微的刺激性作用。鼻中隔穿孔得到有效预防。总之，我们的结果提供了证据，证明脱细胞异种胶原支架适合软骨组织工程。支架很好地整合到鼻中隔软骨缺损中，不会引起强烈的炎症反应，并防止鼻中隔穿孔的发展。因此，我们设想将来有可能将其用于鼻软骨修复。
