Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, People's Republic of China; College of Earth Science, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
J Fish Biol. 2014 Jan;84(1):193-205. doi: 10.1111/jfb.12277. Epub 2013 Dec 23.
To better understand the effects of DNA methylation on the expression patterns of dmrt1 (Doublesex and Mab-3-related transcription factor 1) and cyp19a (Cytochrome P450 19a) in the Japanese flounder Paralichthys olivaceus, quantitative expressions, cellular distributions and cytosine-p-guanine (CpG) methylation patterns of these two genes in the gonads were analysed. The results showed that P. olivaceus dmrt1 expression was 70 times higher in the testis than in the ovary (P < 0·05). Its mRNA was detected clearly in spermatocytes and Sertoli cells of the testis, but weakly in the ovary. Paralichthys olivaceus cyp19a expression was 40 times higher in the ovary than in the testis (P < 0·01). Its mRNA was detected clearly in follicular cells of the ovary, but weakly in spermatocytes of the testis. The dmrt1 promoter CpGs were not methylated in the testis, whereas 57·69% were methylated in the ovary. For the cyp19a promoter CpGs, 97·5% were methylated in the testis and 73·33% were methylated in the ovary. These findings demonstrate that P. olivaceus dmrt1 and cyp19a are sex-related genes with sexual dimorphic expression, CpG methylation levels of the two genes are consistent with their expression quantities, and this epigenetic modification can influence the differential expression of genes in the gonads of P. olivaceus.
为了更好地了解 DNA 甲基化对 dmrt1（双性和 Mab-3 相关转录因子 1）和 cyp19a（细胞色素 P45019a）在日本牙鲆中的表达模式的影响，分析了这两个基因在性腺中的定量表达、细胞分布和胞嘧啶-鸟嘌呤（CpG）甲基化模式。结果表明，dmrt1 在日本牙鲆睾丸中的表达量比卵巢高 70 倍（P<0.05）。其 mRNA 在睾丸的精母细胞和支持细胞中清晰检测到，但在卵巢中较弱。cyp19a 在卵巢中的表达量比在睾丸中高 40 倍（P<0.01）。其 mRNA 在卵巢的滤泡细胞中清晰检测到，但在睾丸的精母细胞中较弱。dmrt1 启动子 CpG 在睾丸中未甲基化，而在卵巢中 57.69%被甲基化。对于 cyp19a 启动子 CpG，97.5%在睾丸中甲基化，73.33%在卵巢中甲基化。这些发现表明，dmrt1 和 cyp19a 是与性别相关的基因，具有性别二态性表达，CpG 甲基化水平与这两个基因的表达量一致，这种表观遗传修饰可以影响日本牙鲆性腺中基因的差异表达。
