Setiawan Iwan, Blanchard G J
Department of Chemistry, Michigan State University , 578 S. Shaw Lane, East Lansing, Michigan 48824, United States.
J Phys Chem B. 2014 Jan 16;118(2):537-46. doi: 10.1021/jp410305m. Epub 2014 Jan 7.
We report on the formation of planar lipid bilayer structures on mica where the bilayer contains the phosphocholine 1,2-dioleoyl-sn-phosphatidylcholine (DOPC), cholesterol, sphingomyelin and sulforhodamine-tagged-1,2-dioleoyl-sn-phosphatidylethanolamine (SR-DOPE). Phase separation is seen for the cholesterol domains within the bilayer structure, and exposure of this supported bilayer to controlled concentrations of ethanol reveals organizational changes on both the micrometer- and molecular-length scales. We report steady state fluorescence imaging, fluorescence lifetime imaging, and fluorescence anisotropy decay imaging for these bilayers. These data are complementary to existing information on the interactions of lipid bilayers with ethanol and point to subtle but important changes in the molecular-scale organization of these structures.
我们报道了在云母上形成平面脂质双层结构，该双层包含磷酸胆碱1,2 - 二油酰基 - sn - 磷脂酰胆碱（DOPC）、胆固醇、鞘磷脂和磺基罗丹明标记的1,2 - 二油酰基 - sn - 磷脂酰乙醇胺（SR - DOPE）。在双层结构内的胆固醇区域可见相分离，并且将这种支撑双层暴露于受控浓度的乙醇中会揭示微米尺度和分子长度尺度上的组织变化。我们报道了这些双层的稳态荧光成像、荧光寿命成像和荧光各向异性衰减成像。这些数据补充了关于脂质双层与乙醇相互作用的现有信息，并指出了这些结构在分子尺度组织上的细微但重要的变化。
