Department of Medical Virology, University of Pretoria, Pretoria, South Africa.
National Health Laboratory Service, Tswhane Academic Division, Pretoria, South Africa.
Trop Med Int Health. 2014 Mar;19(3):293-300. doi: 10.1111/tmi.12251. Epub 2013 Dec 24.
To determine whether gastroenteritis viruses and other enteric viruses could be detected in faecal specimens collected with Bio-wipes.
Faecal specimens, self-collected with Bio-wipes, from 190 individuals (94 diarrhoeal, 93 non-diarrhoeal, 3 unknown) were screened for eight human enteric viruses (enterovirus, hepatitis A virus, adenovirus, astrovirus, norovirus GI and GII, sapovirus and rotavirus) by real-time (reverse transcription)-polymerase chain reaction. Rotaviruses and noroviruses from positive specimens were genotyped.
At least one enteric virus could be detected in 82.6% (157/190) of faecal specimens. Mixed infections of up to four different viruses could be detected in both diarrhoeal and non-diarrhoeal specimens. Enteroviruses were detected most frequently (63.7%), followed by adenoviruses (48.4%) and noroviruses (32.2%). Genotyping was successful for 78.6% of rotaviruses and 44.8% of noroviruses.
Bio-wipes provide a user friendly, easier method for stool collection that facilitates enteric virus detection and genetic characterisation.
确定使用 Bio-wipes 采集的粪便标本中是否可以检测到胃肠炎病毒和其他肠道病毒。
对 190 名个体（94 例腹泻、93 例非腹泻、3 例未知）的粪便标本（使用 Bio-wipes 自行采集）进行了八种人类肠道病毒（肠道病毒、甲型肝炎病毒、腺病毒、星状病毒、诺如病毒 GI 和 GII、肠病毒和轮状病毒）的实时（逆转录）-聚合酶链反应检测。对阳性标本中的轮状病毒和诺如病毒进行了基因分型。
至少有 190 份粪便标本中的 82.6%（157/190）可检测到至少一种肠道病毒。在腹泻和非腹泻标本中均可检测到最多四种不同病毒的混合感染。肠病毒的检出率最高（63.7%），其次是腺病毒（48.4%）和诺如病毒（32.2%）。轮状病毒的基因分型成功率为 78.6%，诺如病毒的基因分型成功率为 44.8%。
Bio-wipes 提供了一种方便用户使用的、更简便的粪便采集方法，有助于肠道病毒的检测和基因特征分析。
