Magwalivha Mpho, Ngandu Jean-Pierre Kabue, Traore Afsatou Ndama, Potgieter Natasha
Department of Biochemistry and Microbiology, Faculty of Science Engineering and Agriculture, University of Venda, Thohoyandou 0950, South Africa.
Adv Virol. 2022 Jun 2;2022:9928378. doi: 10.1155/2022/9928378. eCollection 2022.
Viral diarrhoea is a concern in acute gastroenteritis cases among children younger than 5 years of age. Sapovirus has been noted as an emerging causative agent of acute gastroenteritis worldwide. . The aim of this study was to characterize human sapoviruses targeting the VP1 (NVR and N-terminal) region. Twenty-five samples were randomly selected from 40 sapovirus-positive samples previously detected and analyzed for the VP1 region using the One-Step RT-PCR assay. The PCR products were subjected to Sanger sequencing analysis.
The polyprotein segment (NVR and N-terminal) was successfully amplified from 10/25 samples. Sapovirus GI.1 was the most predominant strain (6/10; 60%), followed by SV-GII.1 (2/10; 20%) and 10% of each GI.3 and GII.3.
Through the partial analysis of the VP1 region, this study provides more data to add on the human sapovirus genetic characterization of circulating strains in South Africa, with the proposition of further analysis of sapovirus VP1 fragments for the viral structure and function.
病毒性腹泻是5岁以下儿童急性胃肠炎病例中的一个关注点。札幌病毒已被确认为全球急性胃肠炎的一种新兴病原体。本研究的目的是对靶向VP1（NVR和N端）区域的人札幌病毒进行特征分析。从先前检测到的40份札幌病毒阳性样本中随机选取25份样本，使用一步法RT-PCR检测法对VP1区域进行分析。PCR产物进行桑格测序分析。
从25份样本中的10份成功扩增出多聚蛋白片段（NVR和N端）。札幌病毒GI.1是最主要的毒株（6/10；60%），其次是SV-GII.1（2/10；20%），GI.3和GII.3各占10%。
通过对VP1区域的部分分析，本研究为南非流行毒株的人札幌病毒基因特征提供了更多数据，并提出进一步分析札幌病毒VP1片段以研究病毒结构和功能。
