Lee H R, Jun H K, Choi B K
Division of High-Risk Pathogen Research, Center for Infectious Diseases, National Institute of Health, Cheongwon-gun, Chungbuk, Korea.
Oral Dis. 2014 Nov;20(8):803-8. doi: 10.1111/odi.12214. Epub 2013 Dec 26.
The aim of this study was to evaluate the effects of Tannerella forsythia and its major surface virulence factor, BspA, on the progression of atherosclerosis in ApoE(-/-) mice and the expression of lipid metabolism-related genes.
PMA-differentiated THP-1 cells were treated with BspA to detect foam cell formation. The proximal aortas of ApoE(-/-) mice injected with T. forsythia or BspA were stained with oil red O to examine lipid deposition. The serum levels of CRP, HDL, and LDL were detected by ELISA. The liver tissue of T. forsythia- or BspA-injected ApoE(-/-) mice was examined for mRNA expression of lipid metabolism-related genes, such as liver X receptors (LXRα and LXRβ) and ATP-binding cassette transporter A1 (ABCA1).
Tannerella forsythia and BspA induced foam cell formation in THP-1 cells and accelerated the progression of atherosclerotic lesions in ApoE(-/-) mice. Mouse serum levels of CRP and LDL were increased, and HDL was decreased by T. forsythia and BspA. The expression levels of LXRα and LXRβ, and ABCA1 in liver tissue were decreased by T. forsythia and BspA.
Tannerella forsythia and BspA augmented atherosclerotic lesion progression in ApoE(-/-) mice. This process may be associated with downregulation of lipid metabolism-related gene expression.
本研究旨在评估具核梭杆菌及其主要表面毒力因子BspA对载脂蛋白E基因敲除（ApoE(-/-)）小鼠动脉粥样硬化进展及脂质代谢相关基因表达的影响。
用BspA处理经佛波酯（PMA）分化的人单核细胞白血病（THP-1）细胞，以检测泡沫细胞形成。给注射了具核梭杆菌或BspA的ApoE(-/-)小鼠的主动脉近端用油红O染色，以检查脂质沉积。通过酶联免疫吸附测定（ELISA）检测血清中C反应蛋白（CRP）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）水平。检测注射具核梭杆菌或BspA的ApoE(-/-)小鼠肝脏组织中脂质代谢相关基因的信使核糖核酸（mRNA）表达，如肝X受体（LXRα和LXRβ）和三磷酸腺苷结合盒转运体A1（ABCA1）。
具核梭杆菌和BspA诱导THP-1细胞形成泡沫细胞，并加速ApoE(-/-)小鼠动脉粥样硬化病变的进展。具核梭杆菌和BspA使小鼠血清中CRP和LDL水平升高，HDL水平降低。具核梭杆菌和BspA使肝脏组织中LXRα、LXRβ和ABCA1的表达水平降低。
具核梭杆菌和BspA加剧了ApoE(-/-)小鼠动脉粥样硬化病变的进展。这一过程可能与脂质代谢相关基因表达的下调有关。
