Delacotte Jérôme, Gourier Christine, Pincet Frédéric
Laboratoire de Physique Statistique, UMR CNRS 8550 associée aux universités Paris 6 et Paris 7, École Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Laboratoire de Physique Statistique, UMR CNRS 8550 associée aux universités Paris 6 et Paris 7, École Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Colloids Surf B Biointerfaces. 2014 May 1;117:545-8. doi: 10.1016/j.colsurfb.2013.11.033. Epub 2013 Dec 7.
Phospholipids are widely used to stabilize oil in water micron size emulsion droplets; the interfacial phospholipid density and tension of such droplets are difficult to estimate. In the present paper, we describe a simple approach by which the measurement of a micron size oil droplet interface fluorescence intensity provides directly both the interfacial phospholipid density and the interfacial tension. This method relies on two prior calibration steps: (i) the quantitative variation of the interfacial tension with fluorescence intensity at droplets interface through micro-manipulation techniques; (ii) the variation of interfacial tension with phospholipid density through monolayer isotherm. Here, we show the validity of this approach with the example of micron size oil droplets stabilized with a phosphatidylcholine phospholipid, in aqueous buffer.
磷脂被广泛用于稳定水包油微米级乳液滴；此类液滴的界面磷脂密度和张力难以估算。在本文中，我们描述了一种简单方法，通过测量微米级油滴界面荧光强度可直接得出界面磷脂密度和界面张力。该方法依赖于两个预先校准步骤：(i) 通过微操作技术使液滴界面处的界面张力随荧光强度发生定量变化；(ii) 通过单分子层等温线使界面张力随磷脂密度发生变化。在此，我们以在水性缓冲液中用磷脂酰胆碱磷脂稳定的微米级油滴为例，展示了该方法的有效性。
