Bear H D
J Surg Res. 1987 Apr;42(4):369-76. doi: 10.1016/0022-4804(87)90171-5.
Spleen cells from late tumor-bearing host mice (TBH) with progressive P815 tumors have been shown to suppress the generation of cytotoxic T lymphocytes (CTL) from spleens of syngeneic immune or early TBH mice in vitro. This suppression is antigen-specific and is mediated by T lymphocytes. By positive selection, a highly enriched population of T cells was obtained from late TBH spleens and fused with the BW5147 HAT-sensitive thymoma. Cloned hybrid cell lines were obtained which released soluble antigen-specific factors capable of suppressing anti-P815 CTL generation. Similar T cell hybrids with suppressive activity were also derived from the fusion of spleen cells of TBHs bearing a HAT-sensitive subline of the P815 tumor.
来自患有进行性P815肿瘤的晚期荷瘤宿主小鼠（TBH）的脾细胞已被证明在体外可抑制同基因免疫小鼠或早期TBH小鼠脾细胞产生细胞毒性T淋巴细胞（CTL）。这种抑制是抗原特异性的，由T淋巴细胞介导。通过阳性选择，从晚期TBH脾中获得了高度富集的T细胞群体，并将其与对HAT敏感的BW5147胸腺瘤融合。获得了克隆的杂交细胞系，其释放出能够抑制抗P815 CTL产生的可溶性抗原特异性因子。具有抑制活性的类似T细胞杂交体也源自携带P815肿瘤HAT敏感亚系的TBH脾细胞的融合。
