Uyttenhove C, Maryanski J, Boon T
J Exp Med. 1983 Mar 1;157(3):1040-52. doi: 10.1084/jem.157.3.1040.
Even though mastocytoma P815 often undergoes a nearly complete rejection in syngeneic mice, the tumor cells almost always escape to form progressive tumors. We found that this was not due to the establishment of an immunosuppressed state because genetically marked P815 cells, that were injected in mice where tumor escape was occurring, were readily rejected. An analysis of escaping tumor cell populations with anti-P815 cytolytic T lymphocyte (CTL) clones showed the presence of stable resistant variants. Using antigen-loss variants found in escaping populations or selected in vitro with CTL clones, we were able to define four different tumor-associated antigenic specificities, each recognized by a specific CTL clone. One of these specificities was absent from all escaping tumor cells and another had been lost by some of them.
尽管肥大细胞瘤P815在同基因小鼠中常常几乎完全被排斥，但肿瘤细胞几乎总是逃脱并形成进行性肿瘤。我们发现这并非由于建立了免疫抑制状态，因为在肿瘤正在发生逃脱的小鼠中注射的基因标记的P815细胞很容易被排斥。用抗P815细胞溶解T淋巴细胞（CTL）克隆对逃脱的肿瘤细胞群体进行分析，结果显示存在稳定的抗性变体。利用在逃脱群体中发现的或用CTL克隆在体外选择的抗原缺失变体，我们能够确定四种不同的肿瘤相关抗原特异性，每种特异性都由一个特定的CTL克隆识别。所有逃脱的肿瘤细胞中都不存在其中一种特异性，而其中一些细胞已经失去了另一种特异性。
