State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, P.R. China; School of Life Science and Technology, UESTC, Chengdu 610054, P.R. China.
State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Optoelectronic Information, University of Electronic Science and Technology of China (UESTC), Chengdu 610054, P.R. China.
Biosens Bioelectron. 2014 May 15;55:157-61. doi: 10.1016/j.bios.2013.11.018. Epub 2013 Dec 1.
A complex thiolated mannose (TM)/quinone functionalised polythiophene (QFPT) thin film was modified on EQCM/Au electrode for recognition of specific carbohydrate-proteins. Different lectins such as those from Sambucus nigra (elder berry), Arachis hypogaea (peanut), Ulex europaeus (gorse, furze), Triticum vulgaris and Concanavalin A (ConA) was used for probes to evaluate bio-sensing performance of the TM/QFPT film. A specific response was observed for ConA from lectins when using the TM/QFPT film as sensing material and employing either elelctrochemical or the QCM method. No response was detected between thiolated mannose and other lectins. The linear relationship between current and ConA concentration is in the range of 0.5-17.5 nM by the elelctrochemical method and the linear relationship between frequency change and ConA concentration is in the range of 0.5-4.5 nM by the QCM method. This shows that the TM/QFPT-modified EQCM biosensor presents a paralleled determination by using electrochemical and the QCM method. The elelctrochemical method of the biosensor can be applicable in a large concentration range and its frequency change can be more precise.
一种复杂的巯基化甘露糖（TM）/醌功能化聚噻吩（QFPT）薄膜被修饰在 EQCM/Au 电极上，用于识别特定的碳水化合物-蛋白质。不同的凝集素，如接骨木（接骨木浆果）、花生（花生）、欧洲荆豆（金雀花，荆豆）、普通小麦和伴刀豆球蛋白 A（ConA）被用作探针，以评估 TM/QFPT 薄膜的生物传感性能。当使用 TM/QFPT 薄膜作为传感材料并采用电化学或 QCM 方法时，从凝集素中观察到 ConA 的特异性响应。巯基化甘露糖与其他凝集素之间未检测到响应。电化学方法中，电流与 ConA 浓度的线性关系在 0.5-17.5 nM 范围内，而 QCM 方法中，频率变化与 ConA 浓度的线性关系在 0.5-4.5 nM 范围内。这表明 TM/QFPT 修饰的 EQCM 生物传感器通过电化学和 QCM 方法呈现出平行的测定。生物传感器的电化学方法可以适用于较大的浓度范围，其频率变化可以更精确。
