Ma Fen, Rehman Abdul, Liu Haiying, Zhang Jingtuo, Zhu Shilei, Zeng Xiangqun
Department of Chemistry, Oakland University , Rochester, Michigan 48309, United States.
Anal Chem. 2015 Feb 3;87(3):1560-8. doi: 10.1021/ac502712q. Epub 2015 Jan 21.
In this report, a new polythiophene interface is fabricated containing fused quinone moieties which are then glycosylated to form a carbohydrate platform for bacterial detection. Very importantly, this interface can be used for label-free and reagentless detection, both by electrochemical and Quartz Crystal Microbalance (QCM) transducers and by using the direct pili-mannose binding as well as Concanavalin A (Con A) mediated lipopolysaccharides (LPS)-mannose binding. The conductive polymer's unique collective properties are very sensitive to very minor perturbations, which result in significant changes of electrical conductivity and providing amplified sensitivity and improved limits of detection (i.e., 25 cell/mL for electrochemical sensor and 50 cells/mL for QCM sensor), a widened logarithmic range of detection (i.e., 3-7 for pili-mannose binding and 2-8 for Con A mediated binding), high specificity and selectivity, and an extraordinary reliability by a mechanism of internal validation. With these analytical performances, the described biosensor is envisaged for being capable of differentiating Gram-negative bacterial strain and species, for many important applications.
在本报告中，制备了一种含有稠合醌部分的新型聚噻吩界面，然后将其糖基化以形成用于细菌检测的碳水化合物平台。非常重要的是，该界面可用于无标记和无试剂检测，既可以通过电化学和石英晶体微天平（QCM）传感器，也可以利用直接的菌毛-甘露糖结合以及伴刀豆球蛋白A（Con A）介导的脂多糖（LPS）-甘露糖结合。导电聚合物独特的集体性质对非常微小的扰动非常敏感，这会导致电导率发生显著变化，并提供增强的灵敏度和改善的检测限（即电化学传感器为25个细胞/毫升，QCM传感器为50个细胞/毫升），拓宽的对数检测范围（即菌毛-甘露糖结合为3至7，Con A介导的结合为2至8），高特异性和选择性，以及通过内部验证机制具有非凡的可靠性。凭借这些分析性能，所描述的生物传感器被设想能够区分革兰氏阴性细菌菌株和物种，适用于许多重要应用。
