AntiCancer, Inc, San Diego, California; Department of Surgery, University of California San Diego, San Diego, California; Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
Department of Surgery, University of California San Diego, San Diego, California.
J Surg Res. 2014 Apr;187(2):510-7. doi: 10.1016/j.jss.2013.11.1083. Epub 2013 Nov 19.
In this study, we investigated the advantages of fluorescence-guided surgery (FGS) in mice of a portable hand-sized imaging system compared with a large fluorescence imaging system or a long-working-distance fluorescence microscope.
Mouse models of human pancreatic cancer for FGS included the following: (1) MiaPaCa-2-expressing green fluorescent protein, (2) BxPC3 labeled with Alexa Fluor 488-conjucated anti-carcinoembryonic antigen (CEA) antibody, and (3) patient-derived orthotopic xenograft (PDOX) labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9 antibody.
Each device could clearly detect the primary MiaPaCa-2-green fluorescent protein tumor and any residual tumor after FGS. In the BxPC3 model labeled with Alexa Fluor 488-conjugated anti-CEA, each device could detect the primary tumor, but the MVX10 could not clearly detect the residual tumor remaining after FGS whereas the other devices could. In the PDOX model labeled with Alexa Fluor 488-conjugated anti-carbohydrate antigen 19-9, only the portable hand-held device could distinguish the residual tumor from the background, and complete resection of the residual tumor was achieved under fluorescence navigation.
The results described in the present report suggest that the hand-held mobile imaging system can be applied to the clinic for FGS because of its convenient size and high sensitivity which should help make FGS widely used.
本研究旨在探讨与大型荧光成像系统或长工作距离荧光显微镜相比，便携式手持成像系统在荧光引导手术（FGS）中应用于小鼠模型的优势。
用于 FGS 的小鼠模型包括以下几种：（1）表达绿色荧光蛋白的 MiaPaCa-2 细胞；（2）用 Alexa Fluor 488 偶联的抗癌胚抗原（CEA）抗体标记的 BxPC3 细胞；（3）用 Alexa Fluor 488 偶联的抗碳水化合物抗原 19-9 抗体标记的患者来源的原位异种移植（PDOX）肿瘤。
每个设备都能清楚地检测到原发性 MiaPaCa-2-绿色荧光蛋白肿瘤和 FGS 后任何残留的肿瘤。在用 Alexa Fluor 488 偶联的抗-CEA 标记的 BxPC3 模型中，每个设备都能检测到原发性肿瘤，但 MVX10 不能清楚地检测到 FGS 后残留的肿瘤，而其他设备可以。在用 Alexa Fluor 488 偶联的抗碳水化合物抗原 19-9 标记的 PDOX 模型中，只有便携式手持设备能够将残留的肿瘤与背景区分开来，并在荧光导航下实现残留肿瘤的完全切除。
本报告中描述的结果表明，由于其便携性和高灵敏度，手持式移动成像系统可应用于 FGS 的临床应用，这有助于使 FGS 得到广泛应用。
