Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.
Department of Oncology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Hum Immunol. 2014 Mar;75(3):239-44. doi: 10.1016/j.humimm.2013.12.006. Epub 2013 Dec 27.
LMP2/LMP7 gene (LMP, low molecular mass protein) perform a critical role in the foreign antigen processing via the major histocompatibility complex-I (MHC-I) complex CD8(+) cytotoxic T lymphocytes (CTL) pathway. This study was designed to investigate whether the sequence variants in LMP2/LMP7 gene would increase the risk of ovarian cancer in the Chinese population. Total of 235 patients with ovarian cancer and 338 normal controls were recruited. Two polymorphisms of LMP2-60 (Arg→His) and LMP7-145 (Gln→Lys) were identified by PCR-RFLP (RFLP, restriction fragment length polymorphism) method. The association of LMP2/LMP7 gene variations with ovarian cancer was assessed by logistic regression analysis. The results revealed that LMP7-145 Gln/Lys and Lys/Lys alleles were associated with the risk of ovarian cancer (P=0.002, OR=2.47; P<0.001, OR=3.23). Meanwhile, the relationship between the LMP7-145 polymorphism and the lymph node metastasis and tumor distant metastasis were also found. No statistical correlation between any of the LMP2-60 polymorphic genotypes and the ovarian cancer clinicopathological characteristics were observed (P>0.05). These results suggested that LMP7 genetic variant could increase the susceptibility to ovarian cancer development; especially increase the risk of lymph node and tumor distant metastasis.
LMP2/LMP7 基因（LMP，低分子量蛋白）通过主要组织相容性复合体-I（MHC-I）复合物 CD8(+)细胞毒性 T 淋巴细胞（CTL）途径在对外来抗原的加工中发挥关键作用。本研究旨在探讨 LMP2/LMP7 基因序列变异是否会增加中国人群患卵巢癌的风险。共招募了 235 名卵巢癌患者和 338 名正常对照者。采用 PCR-RFLP（RFLP，限制性片段长度多态性）方法鉴定 LMP2-60（Arg→His）和 LMP7-145（Gln→Lys）的两个多态性。通过 logistic 回归分析评估 LMP2/LMP7 基因变异与卵巢癌的关联。结果表明，LMP7-145 Gln/Lys 和 Lys/Lys 等位基因与卵巢癌的风险相关（P=0.002，OR=2.47；P<0.001，OR=3.23）。同时，还发现了 LMP7-145 多态性与淋巴结转移和肿瘤远处转移之间的关系。未观察到任何 LMP2-60 多态性基因型与卵巢癌临床病理特征之间存在统计学相关性（P>0.05）。这些结果表明，LMP7 遗传变异可能会增加卵巢癌发展的易感性；特别是增加淋巴结和肿瘤远处转移的风险。
