GABA 茶通过减轻链脲佐菌素诱导的糖尿病大鼠 TNF-α 和 Fas/FasL 介导的细胞凋亡来预防心脏纤维化。

GABA tea prevents cardiac fibrosis by attenuating TNF-alpha and Fas/FasL-mediated apoptosis in streptozotocin-induced diabetic rats.

机构信息

Department of Biotecnology, Hungkuang University, Taichung 43302, Taiwan.

Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan; Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan; Department of Biotechnology, Asia University, Taichung 41354, Taiwan.

出版信息

Food Chem Toxicol. 2014 Mar;65:90-6. doi: 10.1016/j.fct.2013.12.022. Epub 2013 Dec 27.

DOI:10.1016/j.fct.2013.12.022

PMID:24374093

Abstract

GABA tea is a tea product that contains a high level of gamma-aminobutyric acid (GABA). This study investigated the effects of GABA tea on the heart in a diabetic rat model. Male Wistar rats were injected with 55mg/kg streptozotocin (STZ) to induce diabetes for 2weeks and then orally given dosages of 4.55 and 45.5mg/kg/day GABA tea extract for 6weeks. The results revealed that fasting blood glucose levels returned to normal levels in GABA tea-treated diabetic rats, but not in the untreated diabetic rats. Additionally, GABA tea effectively inhibited cardiac fibrosis induced by STZ. Further experiments showed that the STZ-induced protein levels of tumor necrosis factor-alpha (TNF-alpha), Fas, activated caspase-8 and caspase-3 were significantly inhibited by the GABA tea treatment. Therefore, our data suggest that the inhibiting effect of GABA tea on STZ-induced cardiac fibrosis in diabetic rats may be mediated by reducing blood glucose and further attenuating TNF-alpha expression and/or Fas/Fas ligand (FasL)-mediated apoptosis. These findings will provide implications for the potential anti-diabetic properties of GABA tea.

摘要

GABA 茶是一种含有高浓度γ-氨基丁酸（GABA）的茶产品。本研究探讨了 GABA 茶对糖尿病大鼠模型心脏的影响。雄性 Wistar 大鼠注射 55mg/kg 链脲佐菌素（STZ）诱导糖尿病 2 周，然后分别给予 4.55 和 45.5mg/kg/天 GABA 茶提取物 6 周。结果表明，GABA 茶治疗的糖尿病大鼠的空腹血糖水平恢复正常，但未经治疗的糖尿病大鼠则没有。此外，GABA 茶能有效抑制 STZ 诱导的心肌纤维化。进一步的实验表明，GABA 茶处理显著抑制了 STZ 诱导的肿瘤坏死因子-α（TNF-α）、Fas、活化的半胱天冬酶-8 和半胱天冬酶-3 蛋白水平。因此，我们的数据表明，GABA 茶对糖尿病大鼠 STZ 诱导的心肌纤维化的抑制作用可能是通过降低血糖和进一步减弱 TNF-α表达和/或 Fas/Fas 配体（FasL）介导的细胞凋亡来介导的。这些发现将为 GABA 茶的潜在抗糖尿病特性提供依据。