National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.
Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
Antiviral Res. 2014 Mar;103:1-6. doi: 10.1016/j.antiviral.2013.12.008. Epub 2013 Dec 25.
Enterovirus-71 (EV71) is one of the major causative reagents for hand-foot-and-mouth disease. In particular, EV71 causes severe central nervous system infections and leads to numerous dead cases. Although several inactivated whole-virus vaccines have entered in clinical trials, no antiviral agent has been provided for clinical therapy. In the present work, we screened our compound library and identified that suramin, which has been clinically used to treat variable diseases, could inhibit EV71 proliferation with an IC50 value of 40 μM. We further revealed that suramin could block the attachment of EV71 to host cells to regulate the early stage of EV71 infection, as well as affected other steps of EV71 life cycle. Our results are helpful to understand the mechanism for EV71 life cycle and provide a potential for the usage of an approved drug, suramin, as the antiviral against EV71 infection.
肠道病毒 71 型（EV71）是引起手足口病的主要病原体之一。特别是 EV71 可引起严重的中枢神经系统感染，并导致大量死亡病例。尽管已有几种灭活全病毒疫苗进入临床试验阶段，但目前尚无用于临床治疗的抗病毒药物。在本工作中，我们筛选了我们的化合物库，发现已用于治疗多种疾病的苏拉明能够以 40 μM 的 IC50 值抑制 EV71 的增殖。我们进一步揭示，苏拉明可以阻断 EV71 与宿主细胞的附着，从而调控 EV71 感染的早期阶段，同时还影响 EV71 生命周期的其他步骤。我们的结果有助于理解 EV71 生命周期的机制，并为将已批准药物苏拉明用作抗 EV71 感染的抗病毒药物提供了可能性。
