Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC 27695, United States.
Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, NC 27695, United States.
Bioresour Technol. 2014 Feb;153:315-21. doi: 10.1016/j.biortech.2013.11.054. Epub 2013 Nov 27.
The objective of this study was to evaluate the effectiveness of high-frequency focused ultrasound (HFFU) in microalgal cell disruption. Two microalgal species including Scenedesmus dimorphus and Nannochloropsis oculata were treated by a 3.2-MHz, 40-W focused ultrasound and a 100-W, low-frequency (20kHz) non-focused ultrasound (LFNFU). The results demonstrated that HFFU was effective in the disruption of microalgal cells, indicated by significantly increased lipid fluorescence density, the decrease of cell sizes, and the increase of chlorophyll a fluorescence density after treatments. Compared with LFNFU, HFFU treatment was more energy efficient. The combination of high and low frequency treatments was found to be even more effective than single frequency treatment at the same processing time, indicating that frequency played a critical role in cell disruption. In both HFFU and LFNFU treatments, the effectiveness of cell disruption was found to be dependent on the cell treated.
本研究旨在评估高频聚焦超声（HFFU）在微藻细胞破碎中的有效性。采用 3.2MHz、40W 聚焦超声和 100W、低频（20kHz）非聚焦超声（LFNFU）处理两种微藻物种，包括杜氏盐藻和眼点拟微绿球藻。结果表明，HFFU 可有效破碎微藻细胞，处理后脂质荧光密度显著增加，细胞尺寸减小，叶绿素 a 荧光密度增加。与 LFNFU 相比，HFFU 处理的能量效率更高。在相同的处理时间内，发现高低频联合处理比单一频率处理更有效，表明频率在细胞破碎中起着关键作用。在 HFFU 和 LFNFU 处理中，细胞破碎的效果取决于所处理的细胞。
