Rosero Olivér, Németh Károly, Turóczi Zsolt, Fülöp András, Garbaisz Dávid, Győrffy András, Szuák András, Dorogi Bence, Kiss Mátyás, Nemeskéri Ágnes, Harsányi László, Szijártó Attila
1st Department of Surgery, Semmelweis University, 78 Ulloi Street, Budapest, 1082, Hungary,
Surg Today. 2014 Dec;44(12):2345-53. doi: 10.1007/s00595-013-0822-z. Epub 2013 Dec 29.
Rats are the most commonly used animal model for studies of acute lower limb ischemia-reperfusion. The ischemia induced by arterial clamping may cause milder damage than the application of a tourniquet if the presence of a possible collateral system is considered.
Male Wistar rats were randomized into three groups: in group A, the muscle weight affected by ischemia was measured; in group B, the severity of muscle damage caused by the application of a tourniquet and by infrarenal aortic occlusion was examined. Blood and muscle samples were taken from group B to assess the serum necroenzyme, potassium and TNF-α levels, as well as the muscle fiber viability and for histological examinations. In group C, the identification of the lower limb collateral system was performed using corrosion casting.
Tourniquet application affected the lower muscle mass and resulted in significantly more severe injury compared to infrarenal aortic occlusion. This difference was reflected in the serum necroenzyme, potassium and TNF-α levels. The histological examination and viability assay confirmed these findings. The corrosion casts showed several anastomoses capable of supplying the lower limb.
Tourniquet application proved to be capable of inducing absolute lower limb ischemia, in contrast to infrarenal aortic ligation, where a rich collateral system is considered to help mitigate the injury.
大鼠是急性下肢缺血再灌注研究中最常用的动物模型。如果考虑到可能存在的侧支循环系统，动脉夹闭诱导的缺血可能比使用止血带造成的损伤更轻。
将雄性Wistar大鼠随机分为三组：A组测量受缺血影响的肌肉重量；B组检查使用止血带和肾下主动脉闭塞所导致的肌肉损伤严重程度。从B组采集血液和肌肉样本，以评估血清坏死酶、钾和TNF-α水平，以及肌肉纤维活力并进行组织学检查。C组使用铸型腐蚀法鉴定下肢侧支循环系统。
与肾下主动脉闭塞相比，使用止血带影响了下肢肌肉质量，并导致明显更严重的损伤。这种差异反映在血清坏死酶、钾和TNF-α水平上。组织学检查和活力测定证实了这些发现。铸型腐蚀标本显示有几条能够为下肢供血的吻合支。
与肾下主动脉结扎不同，使用止血带能够诱导绝对的下肢缺血，而肾下主动脉结扎时，丰富的侧支循环系统被认为有助于减轻损伤。
