Maksiutov A Z, Eroshkin A M, Kulichkov V A
Mol Biol (Mosk). 1987 Jan-Feb;21(1):39-47.
The effects of amino acid substitutions within the antigenic sites, within the residues close to these sites and within other parts of the molecule on the cross-reaction with the antisera to homologous protein were considered. The method for calculus the values of cross-reactions, based on using primary structures data, X-ray coordinate of one of the homologues and the locations of the antigenic sites is proposed. The values obtained by this method for the cross-reactions of ten myoglobins from various species with antisperm-whale myoglobin sera have a good correlation with the known experimental data. The possibility of using the method to make the location of protein antigenic sites more precise is discussed.
研究了抗原位点内、这些位点附近的残基内以及分子其他部分的氨基酸取代对与同源蛋白抗血清交叉反应的影响。提出了一种基于一级结构数据、同源物之一的X射线坐标和抗原位点位置来计算交叉反应值的方法。通过该方法获得的来自不同物种的十种肌红蛋白与抗抹香鲸肌红蛋白血清交叉反应的值与已知实验数据具有良好的相关性。讨论了使用该方法更精确地定位蛋白质抗原位点的可能性。
