Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Viet Nam.
Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Viet Nam.
Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:704-14. doi: 10.1016/j.saa.2013.11.095. Epub 2013 Nov 25.
Signal processing methods based on the use of derivative, Fourier and wavelet transforms were proposed for the spectrophotometric simultaneous determination of cefoperazone and sulbactam in powders for injection. These transforms were successfully applied to UV spectra and ratio spectra to find suitable working wavelengths. Wavelet signal processing was proved to have distinct advantages (i.e. higher peak intensity obtained, additional smooth function and scaling factor process eliminated) over derivative and Fourier transforms. Especially, a better resolution of spectral overlapping bands was obtained by the use of double signal transform in the sequences such as (i) spectra pre-processed by Fractional Wavelet Transform and subsequently subjected to Continuous Wavelet Transform or Discrete Wavelet Transform, and (ii) derivative - wavelet transforms combined. Calibration graphs for cefoperazone and sulbactam were recorded for the range 10-35 mg/L. Good accuracy and precision were reported for all proposed methods by analyzing synthetic mixtures of cefoperazone and sulbactam. Furthermore, these methods were statistically comparable to RP-HPLC.
基于导数、傅里叶和小波变换的信号处理方法被提出，用于注射用粉末中头孢哌酮和舒巴坦的分光光度同时测定。这些变换成功地应用于紫外光谱和比光谱中，以找到合适的工作波长。小波信号处理被证明具有明显的优势（即获得更高的峰值强度，消除了额外的平滑函数和缩放因子处理），优于导数和傅里叶变换。特别是，通过在序列中使用双信号变换，例如（i）分数小波变换预处理的光谱，随后进行连续小波变换或离散小波变换，和（ii）导数-小波变换的组合，可以获得更好的光谱重叠带分辨率。头孢哌酮和舒巴坦的校准曲线记录在 10-35mg/L 范围内。通过分析头孢哌酮和舒巴坦的合成混合物，报告了所有提出的方法都具有良好的准确性和精密度。此外，这些方法与反相高效液相色谱法具有统计学可比性。
