Parra A, Garcia-Villanova J, Rodenas V, Gomez M D
Servicio de Farmacia, Hospital Virgen del Castillo, Murcia, Spain.
J Pharm Biomed Anal. 1994 May;12(5):653-7. doi: 10.1016/0731-7085(93)e0022-f.
A simple, spectrophotometric assay to measure the concentrations of cefoperazone and sulbactam in injectable formulations is described. Since zero-order spectra are subject to interference, derivative spectrophotometry was used to enhance the spectral details. A linear relationship between derivative amplitudes and the concentrations of the compounds was found. Beer's law is obeyed up to 75 and 80 micrograms ml-1 of cefoperazone in the first and second derivative modes, respectively, and up to 75 micrograms ml-1 of sulbactam in the second derivative mode. Detection limits were 0.64 and 0.88 microgram ml-1, respectively for cefoperazone in the first and second derivative modes and 0.30 micrograms ml-1 for sulbactam in the second derivative mode. The method is rapid, simple, does not require a separation step and has successfully been applied to the assay of commercial injections containing cefoperazone and sulbactam.
本文描述了一种用于测定注射剂中头孢哌酮和舒巴坦浓度的简单分光光度法。由于零级光谱容易受到干扰，因此采用导数分光光度法来增强光谱细节。发现导数幅度与化合物浓度之间存在线性关系。在一阶和二阶导数模式下，头孢哌酮分别在浓度高达75和80微克/毫升时符合比尔定律，在二阶导数模式下，舒巴坦在浓度高达75微克/毫升时符合比尔定律。一阶和二阶导数模式下头孢哌酮的检测限分别为0.64和0.88微克/毫升，二阶导数模式下舒巴坦的检测限为0.30微克/毫升。该方法快速、简单，不需要分离步骤，已成功应用于含头孢哌酮和舒巴坦的商业注射液的测定。
