Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, 45117, Germany.
Virol Sin. 2014 Feb;29(1):33-9. doi: 10.1007/s12250-014-3379-0. Epub 2013 Dec 26.
For subunit vaccines, adjuvants play a key role in shaping the magnitude, persistence and form of targeted antigen-specific immune response. Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application. Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA, RNA, peptides and proteins. Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system, i.e. the cytokine production, was studied. They induced the production of the proinflammatory cytokines IL-8 (Caco-2 cells) and IL-1β (bone marrow-derived macrophages; BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice. The immunostimulation was more pronounced than with free flagellin.
对于亚单位疫苗，佐剂在塑造靶向抗原特异性免疫反应的强度、持久性和形式方面起着关键作用。鞭毛蛋白是一种有效的免疫激活剂，可连接先天炎症反应和适应性免疫，是一种有临床应用前景的佐剂候选物。磷酸钙纳米粒子是不同生物分子（如 DNA、RNA、肽和蛋白质）的有效载体。制备了鞭毛蛋白功能化的磷酸钙纳米粒子，并研究了它们对固有免疫系统（即细胞因子的产生）的免疫刺激作用。它们在体外诱导促炎细胞因子 IL-8（Caco-2 细胞）和 IL-1β（骨髓来源的巨噬细胞；BMDM）的产生，并在小鼠腹腔注射后体内诱导 IL-6 的产生。这种免疫刺激作用比游离鞭毛蛋白更为明显。
