外源性促性腺激素刺激卵巢会降低小鼠囊胚整合素的着床率和表达。
Ovarian stimulation by exogenous gonadotropin decreases the implantation rate and expression of mouse blastocysts integrins.
作者信息
Fayazi Mehri, Beigi Boroujeni Mandana, Salehnia Mojdeh, Khansarinejad Behzad
机构信息
Dept. of Anatomy, Tarbiat Modares University, Tehran, Iran.
出版信息
Iran Biomed J. 2014;18(1):8-15. doi: 10.6091/ibj.1236.2013.
BACKGROUND
Integrins are heterodimeric glycoprotein receptors that regulate the interaction of cells with extracellular matrix and may have a critical role in implantation. The aim of this study was to investigate the effect of ovulation induction on the expression of α4, αv, β1, and β3 integrins in mouse blastocyst at the time of implantation.
METHODS
The ovarian stimulated and non-stimulated pregnant mice were sacrificed on the morning of 5th day of pregnancy. The blastocysts were collected, and the expression of αv, α4, β1, and β3 integrins was examined using real-time RT-PCR and immunocytochemical techniques, then their ovarian hormones were analyzed at the same time. The implantation sites in uterine horns of other pregnant mice in both groups were determined under a stereomicroscope on the 7th day of pregnancy.
RESULTS
The results showed that the expression of αv, β1, and β3 integrins in both mRNA and protein levels was significantly lower in the ovarian stimulated group than the control group, and the maximum ratio of expression was belonged to β1 molecule (P>0.05).
CONCLUSION
The implantation rate in superovulated mice was significantly lower than control mice. It was suggested that ovulation induction decreased the expression of αv, β1, and β3 integrins of mouse blastocysts.
背景
整合素是异二聚体糖蛋白受体，可调节细胞与细胞外基质的相互作用，在着床过程中可能起关键作用。本研究旨在探讨促排卵对小鼠着床期胚泡中α4、αv、β1和β3整合素表达的影响。
方法
在妊娠第5天上午处死经卵巢刺激和未经刺激的妊娠小鼠。收集胚泡，采用实时逆转录聚合酶链反应（RT-PCR）和免疫细胞化学技术检测αv、α4、β1和β3整合素的表达，同时分析其卵巢激素水平。在妊娠第7天，在体视显微镜下确定两组其他妊娠小鼠子宫角的着床部位。
结果
结果显示，卵巢刺激组中αv、β1和β3整合素的mRNA和蛋白水平表达均显著低于对照组，表达比例最高的是β1分子（P>0.05）。
结论
超排卵小鼠的着床率显著低于对照小鼠。提示促排卵降低了小鼠胚泡中αv、β1和β3整合素的表达。
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