Brun P, Dupret J M, Perret C, Thomasset M, Mathieu H
Pediatr Res. 1987 Apr;21(4):362-7. doi: 10.1203/00006450-198704000-00008.
The vitamin D-dependent calcium-binding protein (CaBP) cholecalcin or calbindin, has been used as a molecular marker of 1,25-dihydroxyvitamin D3 action. Mammals possess two CaBPs: a 9,000 mol wt (9K CaBP) and a 28,000 mol wt (28K CaBP). The distinct localization of each protein in the rat has been previously described with the aid of specific radioimmunoassays developed for each CaBP. Antibodies raised against the rat 28K CaBP can be used to detect this protein in a number of mammalian species including humans. In contrast, antibodies against rat 9K CaBP do not cross react with human 9K CaBP, but human 9K CaBP mRNA can be analyzed using a cDNA probe for rat 9K CaBP mRNA. Such a cross-hybridization between the rat cDNA probe and human CaBP mRNA was demonstrated by Northern analysis. We have documented the distribution and evolution of 28K CaBP and 9K CaBP mRNA in human tissues during fetal development from 14 to 32 wk of gestation. 28K CaBP was only present in kidney and cerebellum, and not detectable in duodenum. There was a 2-fold increase of 28K CaBP in the cerebellum between 14 and 24 wk of gestation. The 9K CaBP mRNA was unevenly distributed in human fetal tissues. 9K CaBP mRNA was present in classical vitamin D target tissues such as duodenum and placenta; high levels of 9K CaBP mRNA also were found in thymus and lung.(ABSTRACT TRUNCATED AT 250 WORDS)
维生素D依赖性钙结合蛋白（CaBP）胆钙化蛋白或钙结合蛋白，已被用作1,25 - 二羟基维生素D3作用的分子标志物。哺乳动物有两种CaBP：一种分子量为9000（9K CaBP），另一种分子量为28000（28K CaBP）。借助为每种CaBP开发的特异性放射免疫测定法，先前已描述了大鼠体内每种蛋白质的不同定位。针对大鼠28K CaBP产生的抗体可用于在包括人类在内的多种哺乳动物物种中检测该蛋白。相比之下，针对大鼠9K CaBP的抗体与人9K CaBP不发生交叉反应，但可使用大鼠9K CaBP mRNA的cDNA探针分析人9K CaBP mRNA。通过Northern分析证实了大鼠cDNA探针与人CaBP mRNA之间的这种交叉杂交。我们记录了妊娠14至32周胎儿发育期间人组织中28K CaBP和9K CaBP mRNA的分布及演变。28K CaBP仅存在于肾脏和小脑中，在十二指肠中未检测到。在妊娠14至24周期间，小脑中的28K CaBP增加了2倍。9K CaBP mRNA在人胎儿组织中分布不均。9K CaBP mRNA存在于经典的维生素D靶组织如十二指肠和胎盘中；在胸腺和肺中也发现了高水平的9K CaBP mRNA。（摘要截短于250字）
