Thomasset M, Perret C, Brehier A, Balmain N, Cuisinier-Gleizes P, Mathieu H
Arch Fr Pediatr. 1985 Mar;42(3):231-6.
Some applications to man of specific markers of the molecular action of vitamin D (1.25(OH)2D3 receptors and antibodies to hormone-dependent proteins (CaBP and cDNA] are reported in this study. On case of type II vitamin-dependent rickets was characterized by 1.25(OH)2D3 plasma level greater than 250 pg/ml and a ten-fold decrease of the number of binding sites of the hormone in cultured skin fibroblasts. We propose that CaBP 28K and/or 9K-containing cells, such as Purkinje's cells and chondroblasts may be targets for vitamin D action. Detection in fetuses, from the 20th week of gestation, of CaBP 9K messenger RNA in the duodenum and sternum and presence of CaBP 28K and 9K in the chondroblasts of the upper extremity of tibia, suggest that vitamin D acts on the nucleus of its target-cells during fetal development. Finally, discovery of the gene of CaBP 9K in man opens the prospect of studies which will improve the understanding of the mechanism of action of vitamin D.
本研究报道了维生素D分子作用的特定标志物（1,25-二羟维生素D3受体以及激素依赖性蛋白（钙结合蛋白和互补DNA）的抗体）在人体中的一些应用。在II型维生素依赖性佝偻病病例中，其特征为血浆1,25-二羟维生素D3水平高于250皮克/毫升，且培养的皮肤成纤维细胞中激素结合位点数量减少了十倍。我们提出，含28K和/或9K钙结合蛋白的细胞，如浦肯野细胞和成软骨细胞，可能是维生素D作用的靶点。在妊娠20周起的胎儿十二指肠和胸骨中检测到9K钙结合蛋白信使核糖核酸，以及在胫骨上端成软骨细胞中存在28K和9K钙结合蛋白，这表明维生素D在胎儿发育过程中作用于其靶细胞的细胞核。最后，人类9K钙结合蛋白基因的发现为深入了解维生素D作用机制的研究开辟了前景。
