Xu Bin, Peng Min, Song Qibin
Oncology Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, People's Republic of China.
Tumour Biol. 2014 May;35(5):4087-93. doi: 10.1007/s13277-013-1534-0. Epub 2013 Dec 28.
Recently, telomerase-targeted therapy was studied intensively; however, many studies have verified the existence of alternative lengthening mechanisms of telomeres in vitro. In the present work, we explored the expression characteristic of the two kinds of telomere-prolonging mechanisms in the breast cancer tissues per se. Furthermore, we studied the relationship between Her-2 expression and ALT pathway. Ninety samples of breast cancer tissues were examined in this research. RT-PCR was used for the detection of the expression of human telomerase reverse transcriptase (hTERT); IHC was used for the detection of the expression of promyelocytic leukemia body (PML) bodies; the co-expression of PML bodies and hTERT was detected using the QDs-based immunofluorescence. The co-expression of PML body and hTERT was found in the same cell in breast cancer tissues, and ten samples expressed neither PML bodies nor hTERT. Additionally, the expression of PML bodies and Her-2 was statistically co-related (P = 0.047). The two kinds of mechanisms of telomere extension can co-exist in the same cell in beast cancer tissues, and there may be other mechanisms of telomere extension functioning in human breast carcinoma.
最近，针对端粒酶的治疗方法得到了深入研究；然而，许多研究已经在体外证实了端粒存在替代延长机制。在本研究中，我们探讨了这两种端粒延长机制在乳腺癌组织本身中的表达特征。此外，我们研究了Her-2表达与ALT途径之间的关系。本研究检测了90份乳腺癌组织样本。采用RT-PCR检测人端粒酶逆转录酶（hTERT）的表达；采用免疫组化法检测早幼粒细胞白血病小体（PML小体）的表达；利用基于量子点的免疫荧光检测PML小体与hTERT的共表达。在乳腺癌组织的同一细胞中发现了PML小体与hTERT的共表达，10份样本既不表达PML小体也不表达hTERT。此外，PML小体与Her-2的表达在统计学上具有相关性（P = 0.047）。端粒延长的两种机制可在乳腺癌组织的同一细胞中共存，并且在人类乳腺癌中可能存在其他端粒延长机制发挥作用。
