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RNA末端标记和RNA连接酶活性可在锥虫的全细胞提取物中产生环状核糖体RNA。

RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.

作者信息

White T C, Borst P

出版信息

Nucleic Acids Res. 1987 Apr 24;15(8):3275-90. doi: 10.1093/nar/15.8.3275.

DOI:10.1093/nar/15.8.3275
PMID:2437529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340729/
Abstract

We have found two enzymatic activities in whole cell extracts from Trypanosoma brucei; an end-labeling reaction involving a single uridine at the 3' end of ribosomal RNAs (rRNAs) and an RNA ligase activity joining 5' monophosphates to 3' hydroxyl groups. The RNA ligase acts upon one of the small rRNAs (180 nucleotides) from the trypanosome ribosomal repeat unit, forming a circular RNA. The specific circularization of this small rRNA is probably dependent on the secondary structure of the molecule and is not detectable in vivo.

摘要

我们在布氏锥虫的全细胞提取物中发现了两种酶活性；一种是涉及核糖体RNA（rRNA）3'端单个尿苷的末端标记反应，另一种是将5'单磷酸与3'羟基连接的RNA连接酶活性。该RNA连接酶作用于锥虫核糖体重复单元中的一种小rRNA（180个核苷酸），形成环状RNA。这种小rRNA的特异性环化可能取决于分子的二级结构，在体内无法检测到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/68eb9991ac6d/nar00252-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/1d70864eb2ef/nar00252-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/0183b4ba33c4/nar00252-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/2528855b9984/nar00252-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/68eb9991ac6d/nar00252-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/1d70864eb2ef/nar00252-0089-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/0183b4ba33c4/nar00252-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/2528855b9984/nar00252-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df13/340729/68eb9991ac6d/nar00252-0097-a.jpg

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本文引用的文献

1
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.显微注射非洲爪蟾卵母细胞中的RNA加工。剪接转运RNA中碱基修饰的顺序添加。
J Mol Biol. 1981 Jan 15;145(2):405-20. doi: 10.1016/0022-2836(81)90212-6.
2
Primary structures of four novel small ribosomal RNAs from Crithidia fasciculata.来自纤细短膜虫的四种新型小核糖体RNA的一级结构。
Can J Biochem Cell Biol. 1983 Jan;61(1):38-45. doi: 10.1139/o83-006.
3
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
布氏锥虫RNA编辑复合体的两种RNA连接酶：克隆必需的条带IV基因并鉴定条带V基因。
Mol Cell Biol. 2001 Feb;21(4):979-89. doi: 10.1128/MCB.21.4.979-989.2001.
4
Trypanosoma brucei guide RNA poly(U) tail formation is stabilized by cognate mRNA.布氏锥虫向导RNA的聚尿苷酸尾形成由同源mRNA稳定。
Mol Cell Biol. 2000 Feb;20(3):883-91. doi: 10.1128/MCB.20.3.883-891.2000.
5
RNA editing in kinetoplastid protozoa.动质体原生动物中的RNA编辑。
Microbiol Mol Biol Rev. 1997 Mar;61(1):105-20. doi: 10.1128/mmbr.61.1.105-120.1997.
6
Uridine insertion into preedited mRNA by a mitochondrial extract from Leishmania tarentolae: stereochemical evidence for the enzyme cascade model.利用来自热带利什曼原虫的线粒体提取物将尿苷插入到编辑前的mRNA中：酶级联模型的立体化学证据
Mol Cell Biol. 1996 Aug;16(8):4584-9. doi: 10.1128/MCB.16.8.4584.
7
Characterization of a RNA virus from the parasite Leishmania.
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5979-82. doi: 10.1073/pnas.86.15.5979.
8
In vitro 3' end processing and poly(A) tailing of RNA in Trypanosoma cruzi.
Nucleic Acids Res. 1989 Jun 26;17(12):4647-60. doi: 10.1093/nar/17.12.4647.
9
RNA editing and the mitochondrial cryptogenes of kinetoplastid protozoa.动质体原生动物的RNA编辑与线粒体隐蔽基因
Cell. 1989 May 5;57(3):355-66. doi: 10.1016/0092-8674(89)90911-2.
10
A 5' exo-ribonuclease and RNA ligase of T. brucei.布氏锥虫的一种5'外切核糖核酸酶和RNA连接酶。
Nucleic Acids Res. 1988 Oct 25;16(20):9737-59. doi: 10.1093/nar/16.20.9737.
HeLa细胞提取物中的RNA 3'-末端磷酸环化酶活性和RNA连接
Nucleic Acids Res. 1983 Mar 11;11(5):1405-18. doi: 10.1093/nar/11.5.1405.
4
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Eur J Biochem. 1980 Jun;107(2):303-14. doi: 10.1111/j.1432-1033.1980.tb06030.x.
5
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Nucleic Acids Res. 1982 Dec 11;10(23):7521-9. doi: 10.1093/nar/10.23.7521.
6
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Nucleic Acids Res. 1984 Dec 21;12(24):9457-72. doi: 10.1093/nar/12.24.9457.
7
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Nucleic Acids Res. 1981 Dec 21;9(24):6953-8. doi: 10.1093/nar/9.24.6953.
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J Mol Biol. 1983 Jun 5;166(4):537-56. doi: 10.1016/s0022-2836(83)80283-6.
9
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Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8. doi: 10.1073/pnas.79.5.1474.
10
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Nucleic Acids Res. 1981 Jun 11;9(11):2433-53. doi: 10.1093/nar/9.11.2433.