• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过来自小麦胚芽和衣藻的一种新型RNA连接酶，经由2'-磷酸单酯、3',5'-磷酸二酯键实现线性类病毒RNA的环化。

Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.

作者信息

Kikuchi Y, Tyc K, Filipowicz W, Sänger H L, Gross H J

出版信息

Nucleic Acids Res. 1982 Dec 11;10(23):7521-9. doi: 10.1093/nar/10.23.7521.

DOI:10.1093/nar/10.23.7521
PMID:6760127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327027/
Abstract

A novel type of RNA ligase activity in extracts of wheat germ or Chlamydomonas requires 2', 3'-cyclic phosphate and 5'-phosphate ends for ligation to form a 2'-phosphomonoester, 3',5'-phosphodiester bond. Using 5'-3 2P-labeled linear PSTV, we demonstrate that RNase T1-nicked viroid predominantly forms (formula; see text) U-bonds. Natural linear PSTV, however, forms mainly (formula; see text) A-bonds upon enzymatic circularization. We show that natural linear PSTV RNA has nicks between C181 and A182, or between C348 and A349, and that consequently C181 and C348 carry 2',3'-cyclophosphate termini.

摘要

小麦胚芽或衣藻提取物中的一种新型RNA连接酶活性，在连接形成2'-磷酸单酯、3',5'-磷酸二酯键时需要2',3'-环磷酸和5'-磷酸末端。使用5'-3 2P标记的线性马铃薯纺锤块茎类病毒（PSTV），我们证明核糖核酸酶T1切割的类病毒主要形成（分子式；见正文）U键。然而，天然线性PSTV在酶促环化时主要形成（分子式；见正文）A键。我们表明天然线性PSTV RNA在C181和A182之间或C348和A349之间有切口，因此C181和C348带有2',3'-环磷酸末端。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/8ae7e83d28a3/nar00392-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/0473086d6224/nar00392-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/dceb1b484342/nar00392-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/35e87cf5b8dc/nar00392-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/8ae7e83d28a3/nar00392-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/0473086d6224/nar00392-0071-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/dceb1b484342/nar00392-0072-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/35e87cf5b8dc/nar00392-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417c/327027/8ae7e83d28a3/nar00392-0075-a.jpg

相似文献

1
Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.通过来自小麦胚芽和衣藻的一种新型RNA连接酶，经由2'-磷酸单酯、3',5'-磷酸二酯键实现线性类病毒RNA的环化。
Nucleic Acids Res. 1982 Dec 11;10(23):7521-9. doi: 10.1093/nar/10.23.7521.
2
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.小麦胚芽中一种新型RNA连接酶形成2'-磷酸单酯、3',5'-磷酸二酯键。
Nature. 1981;293(5828):112-6. doi: 10.1038/293112a0.
3
Enzymatic mechanism of an RNA ligase from wheat germ.来自小麦胚芽的一种RNA连接酶的酶促机制。
J Biol Chem. 1983 Jul 10;258(13):8374-83.
4
An RNA ligase from wheat germ which participates in transfer RNA splicing in vitro.一种来自小麦胚芽的RNA连接酶，其在体外参与转运RNA剪接。
J Biol Chem. 1983 Jul 10;258(13):8365-73.
5
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.通过2'-磷酸单酯、3',5'-磷酸二酯键进行RNA连接：2',3'-环磷酸末端的要求以及5'-羟基多核苷酸激酶的参与
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8. doi: 10.1073/pnas.79.5.1474.
6
Isolation and characterization of RNA ligase from wheat germ.从小麦胚芽中分离并鉴定RNA连接酶。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3933-7. doi: 10.1073/pnas.80.13.3933.
7
Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity.小麦胚芽RNA连接酶的纯化。I. 与连接酶相关的5'-羟基多核苷酸激酶活性的特性
J Biol Chem. 1986 May 25;261(15):6684-93.
8
Purification of wheat germ RNA ligase. II. Mechanism of action of wheat germ RNA ligase.小麦胚芽RNA连接酶的纯化。II. 小麦胚芽RNA连接酶的作用机制。
J Biol Chem. 1986 May 25;261(15):6694-704.
9
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.HeLa细胞提取物中的RNA 3'-末端磷酸环化酶活性和RNA连接
Nucleic Acids Res. 1983 Mar 11;11(5):1405-18. doi: 10.1093/nar/11.5.1405.
10
Monomeric linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication.柑橘裂皮病类病毒在体内加工产生的单体线性RNA具有5'-磷酸单酯和3'-羟基末端：对参与复制的核糖核酸酶和RNA连接酶的影响。
J Virol. 2008 Oct;82(20):10321-5. doi: 10.1128/JVI.01229-08. Epub 2008 Aug 13.

引用本文的文献

1
Traversing the RNA world.穿越RNA世界。
J Biol Chem. 2017 May 19;292(20):8122-8135. doi: 10.1074/jbc.X117.789065. Epub 2017 Apr 5.
2
Relationship of viroids and certain other plant pathogenic nucleic acids to group I and II introns.类病毒与某些其它植物病原核酸与 I 组和 II 组内含子的关系。
Plant Mol Biol. 1986 Mar;7(2):129-42. doi: 10.1007/BF00040139.
3
Monomeric linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication.

本文引用的文献

1
Cell-free circularization of viroid progeny RNA by an RNA ligase from wheat germ.小麦胚 RNA 连接酶对类病毒前体 RNA 的无细胞环化作用。
Science. 1982 Sep 17;217(4565):1147-9. doi: 10.1126/science.217.4565.1147.
2
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
3
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
柑橘裂皮病类病毒在体内加工产生的单体线性RNA具有5'-磷酸单酯和3'-羟基末端：对参与复制的核糖核酸酶和RNA连接酶的影响。
J Virol. 2008 Oct;82(20):10321-5. doi: 10.1128/JVI.01229-08. Epub 2008 Aug 13.
4
Processing of nuclear viroids in vivo: an interplay between RNA conformations.体内核酶的加工：RNA构象之间的相互作用
PLoS Pathog. 2007 Nov;3(11):e182. doi: 10.1371/journal.ppat.0030182.
5
Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts.莱茵衣藻叶绿体中日光病毒科RNA的加工
J Virol. 2007 Apr;81(8):4363-6. doi: 10.1128/JVI.02556-06. Epub 2007 Feb 7.
6
Evidence for a single rolling circle in the replication of potato spindle tuber viroid.马铃薯纺锤块茎类病毒复制中单滚环的证据。
Proc Natl Acad Sci U S A. 1988 Dec;85(23):9128-32. doi: 10.1073/pnas.85.23.9128.
7
Linear oligomeric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells.线性寡聚马铃薯纺锤块茎类病毒（PSTV）RNA 在与来自健康马铃薯细胞的核提取物孵育时，在体外被准确加工成单体环状类病毒。
EMBO J. 1987 Aug;6(8):2173-83. doi: 10.1002/j.1460-2075.1987.tb02488.x.
8
Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.通过 2'-磷酸单酯、3'，5'-磷酸二酯键将内源性 tRNA 3' 半分子连接到其相应的 5' 半分子，在衣藻提取物中。
EMBO J. 1983;2(4):605-10. doi: 10.1002/j.1460-2075.1983.tb01470.x.
9
Plant tRNA ligases are multifunctional enzymes that have diverged in sequence and substrate specificity from RNA ligases of other phylogenetic origins.植物tRNA连接酶是多功能酶，其在序列和底物特异性上与其他系统发育起源的RNA连接酶有所不同。
Nucleic Acids Res. 2005 Jan 14;33(1):388-99. doi: 10.1093/nar/gki174. Print 2005.
10
Junction phosphate is derived from the precursor in the tRNA spliced by the archaeon Haloferax volcanii cell extract.连接磷酸是由嗜盐嗜碱古菌沃氏嗜盐菌细胞提取物剪接的tRNA前体衍生而来。
RNA. 2000 Jul;6(7):1019-30. doi: 10.1017/s1355838200000613.
小麦胚芽中一种新型RNA连接酶形成2'-磷酸单酯、3',5'-磷酸二酯键。
Nature. 1981;293(5828):112-6. doi: 10.1038/293112a0.
4
Rapid cataloging of ribonuclease T1 resistant oligonucleotides from ribosomal RNAs for phylogenetic studies.用于系统发育研究的核糖体RNA中核糖核酸酶T1抗性寡核苷酸的快速编目。
J Mol Evol. 1981;17(4):227-36. doi: 10.1007/BF01732760.
5
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.通过2'-磷酸单酯、3',5'-磷酸二酯键进行RNA连接：2',3'-环磷酸末端的要求以及5'-羟基多核苷酸激酶的参与
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1474-8. doi: 10.1073/pnas.79.5.1474.
6
Absence of the sequence G-T-psi-C-G(A)- in several eukaryotic cytoplasmic initiator transfer RNAs.几种真核细胞质起始转运核糖核酸中不存在G-T-假尿嘧啶-C-G(A)序列。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1041-5. doi: 10.1073/pnas.70.4.1041.
7
Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.类病毒是单链共价闭合环状RNA分子，以高度碱基配对的棒状结构形式存在。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3852-6. doi: 10.1073/pnas.73.11.3852.
8
Nucleotide sequence and secondary structure of potato spindle tuber viroid.马铃薯纺锤块茎类病毒的核苷酸序列与二级结构
Nature. 1978 May 18;273(5659):203-8. doi: 10.1038/273203a0.
9
Replicative form of Semliki Forest virus RNA contains an unpaired guanosine.塞姆利基森林病毒RNA的复制形式含有一个未配对的鸟苷。
Nature. 1979 Dec 13;282(5740):754-6. doi: 10.1038/282754a0.
10
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.体外32P标记在非放射性tRNA序列分析中的应用。
Methods Enzymol. 1979;59:58-109. doi: 10.1016/0076-6879(79)59072-7.