Duša Filip, Slais Karel
Institute of Analytical Chemistry of Academy of Sciences of the Czech Republic, v. v. i., Brno, Czech Republic; Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Electrophoresis. 2014 Apr;35(8):1114-7. doi: 10.1002/elps.201300518. Epub 2014 Feb 7.
The power supply for IEF based on features of the Cockcroft-Walton voltage multiplier (CW VM) is described in this work. The article describes a design of the IEF power supply, its electric characteristics, and testing by IEF analysis. A circuit diagram of the power supply included two opposite charged branches (each consisting of four voltage doublers). The designed CW VM was powered by 230 V/50 Hz alternate current and it generated up to 5 kV and 90 mW at the output. Voltage and current characteristics of the power supply were measured by known load resistances in the range from 10 kΩ to 1 GΩ, which is a common resistance range for IEF strip geometry. Further, the power supply was tested by a separation of a model mixture of colored pI markers using a 175 × 3 × 0.5 mm focusing bed. Automatically limited power load enabled analysis of samples without previous optimization of the focusing voltage or electric current time courses according to sample composition. Moreover, the developed power supply did not produce any intrinsic heat and was easy to set up with cheap and commonly available parts.
本文描述了基于考克饶夫-瓦尔顿倍压器（CW VM）特性的等电聚焦（IEF）电源。文章介绍了IEF电源的设计、电气特性以及通过IEF分析进行的测试。该电源的电路图包括两个相反带电分支（每个分支由四个倍压器组成）。所设计的CW VM由230 V/50 Hz交流电供电，其输出可产生高达5 kV的电压和90 mW的功率。通过10 kΩ至1 GΩ范围内的已知负载电阻测量电源的电压和电流特性，这是IEF条带几何形状的常见电阻范围。此外，使用175×3×0.5 mm聚焦床对彩色pI标记物的模型混合物进行分离，对电源进行了测试。自动限制功率负载使得无需根据样品组成预先优化聚焦电压或电流时间进程就能对样品进行分析。此外，所开发的电源不会产生任何固有热量，并且易于使用廉价且常见的部件进行设置。
