Bioprocess Engineering Research Group, Biotechnology Research Institute, Universiti Malaysia Sabah, Sabah, Malaysia.
J Sep Sci. 2014 Mar;37(5):455-64. doi: 10.1002/jssc.201300995. Epub 2014 Jan 16.
To date, the number of published reports on the large-volume preparation of polymer-based monolithic chromatography adsorbents is still lacking and is of great importance. Many critical factors need to be considered when manufacturing a large-volume polymer-based monolith for chromatographic applications. Structural integrity, validity, and repeatability are thought to be the key factors determining the usability of a large-volume monolith in a separation process. In this review, we focus on problems and solutions pertaining to heat dissipation, pore size distribution, "wall channel" effect, and mechanical strength in monolith preparation. A template-based method comprising sacrificial and nonsacrificial techniques is possibly the method of choice due to its precise control over the porous structure. However, additional expensive steps are usually required for the template removal. Other strategies in monolith preparation are also discussed.
迄今为止，关于大体积聚合物整体柱色谱吸附剂的大量制备的报道仍然很少，这一点非常重要。在制造用于色谱应用的大体积聚合物整体柱时，需要考虑许多关键因素。结构完整性、有效性和可重复性被认为是决定大体积整体柱在分离过程中可用性的关键因素。在这篇综述中，我们重点讨论了在整体柱制备过程中涉及到的散热、孔径分布、“壁通道”效应和机械强度等问题及其解决方案。基于模板的方法包括牺牲和非牺牲技术，由于其对多孔结构的精确控制，可能是首选方法。但是，通常需要额外的昂贵步骤来去除模板。本文还讨论了其他整体柱制备策略。
